Disruption of non-classically secreted protein(MoMtp)compromised conidiation,stress homeostasis,and pathogenesis of Magnaporthe oryzae  

作　　者：Wajjiha Batool Justice Norvienyeku Wei Yi Zonghua Wang Shihong Zhang Lili Lin 

机构地区：[1]Key Laboratory for Extreme-Environmental Microbiology,College of Plant Protection,Shenyang Agricultural University,Shenyang 110866,China [2]Ministerial and Provincial Joint Innovation Center for Safety Production of Cross-Strait Crops,College of Life Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China [3]Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests,College of Plant Protection,Hainan University,Haikou 570288,China

出　　处：《Journal of Integrative Agriculture》2024年第8期2686-2702,共17页农业科学学报（英文版）

基　　金：funded by the National Natural Science Foundation of China(32172364 to Shihong Zhang and 32272513 to Zonghua Wang);Fujian Agriculture and Forestry University scholarship,China for Wajjiha Batool。

摘　　要：Blast disease,caused by the hemibiotrophic ascomycete fungus,Magnaporthe oryzae,is a significant threat to sustainable rice production worldwide.Studies have shown that the blast fungus secretes vast arrays of functionally diverse proteins into the host cell for a successful disease progression.However,the final destinations of these effector proteins inside the host cell and their role in advancing fungal pathogenesis remain a mystery.Here,we reported that a putative mitochondrial targeting non-classically secreted protein(MoMtp)positively regulates conidiogenesis and appressorium maturation in M.oryzae.Moreover,MoM TP gene deletion mutant strains triggered a hypersensitive response when inoculated on rice leaves displaying that MoMtp is essential for the virulence of M.oryzae.In addition,cell wall and oxidative stress results indicated that MoMtp is likely involved in the maintenance of the structural integrity of the fungus cell.Our study also demonstrates an upregulation in the expression pattern of the MoMTP gene at all stages of infection,indicating its possible regulatory role in host invasion and the infectious development of M.oryzae.Furthermore,Agrobacterium infiltration and sheath inoculation confirmed that MoMtpGFP protein is predominantly localized in the host mitochondria of tobacco leaf and rice cells.Taken together,we conclude that MoMtp protein likely promotes the normal conidiation and pathogenesis of M.oryzae and might have a role in disturbing the proper functioning of the host mitochondria during pathogen invasion.

关 键 词：plant–pathogen interactions EFFECTORS hypersensitive response PATHOGENESIS MITOCHONDRIA 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]