The Ca^(2+)/CaN/ACC and cAMP/PKA/HK signal pathways are required for PBAN-mediated sex pheromone biosynthesis in Conogethes punctiferalis  

作　　者：Yao Zhang Zelong She Ruolan He Shuangyan Yao Xiang Li Xiaoguang Liu Xinming Yin Jizhen Wei Mengfang Du Shiheng An 

机构地区：[1]State Key Laboratory of Wheat and Maize Crop Science/Henan International Laboratory for Green Pest Control/College of Plant Protection,Henan Agricultural University,Zhengzhou 450046,China

出　　处：《Journal of Integrative Agriculture》2024年第8期2735-2751,共17页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31970472,32272547);the National Science Fund of Henan Province for Distinguished Young Scholars,China(202300410191);the Basic Research Project of the Key Scientific Research Projects of Universities in Henan Province,China(21zx013);the Henan Agricultural Research System,China(HARS-2209-G3);the Henan Special Support for High-Level Talents Central Plains Science and Technology Innovation Leading Talents,China(224200510018);the earmarked fund for China Agricultural Research System(CARS-27)。

摘　　要：Conogethes punctiferalis is a crop and fruit pest that has caused serious economic losses to agricultural production.This pest relies heavily on its sex pheromone to ensure sexual encounters and subsequent mating success.However,the molecular mechanism underlying sex pheromone biosynthesis in this species remains elusive.The present study investigated the detailed mechanism underlying PBAN-regulated sex pheromone biosynthesis in C.punctiferalis by transcriptome sequencing of the C.punctiferalis pheromone glands(PGs)and subsequent functional identification of the target genes.The results showed that female mating started from the first scotophase,and peaked at the second to fifth scotophases in accordance with the release of sex pheromones.PBAN regulated sex pheromone biosynthesis by employing Ca^(2+)and cAMP as secondary messengers,as demonstrated by RNA interference(RNAi),pharmacological inhibitors,and behavioral assays.Further investigation revealed that calcineurin(CaN)and acetyl-CoA carboxylase(ACC)were activated by PBAN/Ca^(2+)signaling,and the RNAimediated knockdown of CaN and ACC transcripts significantly reduced sex pheromone production,ultimately leading to a significantly reduced ability of females to attract males.Importantly,hexokinase(HK)was found to regulate sex pheromone biosynthesis in response to the PBAN/cAMP/PKA signaling pathway,as demonstrated by RNAi,enzyme activity,and pharmacological inhibitor assays.Furthermore,Far2 and Desaturase1 were found to participate in PBAN-regulated sex pheromone biosynthesis.Altogether,our findings revealed that PBAN regulates sex pheromone biosynthesis through the PBANR/Ca^(2+)/CaN/ACC and PBANR/cAMP/PKA/HK pathways in C.punctiferalis,which enriches our comprehension of the details of sex pheromone biosynthesis in moths.

关 键 词：Conogethes punctiferalis sex pheromone CAMP/PKA CALCINEURIN acetyl-CoA carboxylase HEXOKINASE 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]