The synergistic regulatory effect of PTP1B and PTK inhibitors on the development of Oedaleus decorus asiaticus Bei-Bienko  

作　　者：Shuang Li Sibo Liu Chaomin Xu Shiqian Feng Xiongbing Tu Zehua Zhang 

机构地区：[1]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China [2]Chongqing Academy of Agricultural Sciences,Chongqing 401329,China [3]Institute of Grassland Research,Chinese Academy of Agricultural Sciences,Hohhot 010010,China [4]Inner Mongolia University,Hohhot 010019,China [5]Scientific Observation and Experimental Station of Pests in Xilin Gol Rangeland,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Xilinhot 026000,China

出　　处：《Journal of Integrative Agriculture》2024年第8期2752-2763,共12页农业科学学报（英文版）

基　　金：the National Key R&D Program of China(2022YFD1400500);the earmarked fund for China Agriculture Research System(CARS-34);the Central Public-interest Scientific Institution Basal Research Fund of China(S2022XM21)。

摘　　要：Tyrosine phosphorylation is crucial for controlling normal cell growth,survival,intercellular communication,gene transcription,immune responses,and other processes.protein tyrosine phosphatase(PTP)and protein tyrosine kinases(PTK)can achieve this goal by regulating multiple signaling pathways.Oedaleus decorus asiaticus is an important pest that infests the Mongolian Plateau grassland.We aimed to evaluate the survival rate,growth rate,overall performance,and ovarian developmental morphology of the 4th instar nymphs of O.decorus asiaticus while inhibiting the activity of protein tyrosine phosphatase-1B(PTP1B)and PTK.In addition,the expression and protein phosphorylation levels of key genes in the MAPK signaling pathway and antioxidant enzyme activity were assessed.The results showed no significant differences in survival rate,growth rate,or overall performance between PTP1B inhibitor treatment and control.However,after PTK inhibitor treatment,these indexes were significantly lower than those in the control.The ovarian size of female larvae after 15 days of treatment with PTK inhibitors showed significantly slower development,while female larvae treated with PTP1B exhibited faster ovarian growth than the control group.In comparison to controls and nymphs treated with PTK inhibitors,the expression and phosphorylation levels of key genes in the MAPK signaling pathway under PTP1B inhibitor treatments were significantly higher in 4th instar nymphs.However,reactiveoxygen(ROS)species levels and the activities of NADPH oxidase and other antioxidant enzymes were considerably reduced,although they were significantly greater in the PTK inhibitor treatment.The results suggest that PTP1B and PTK feedback inhibition in the mitogen-activated-protein kinases(MAPK)signal transfer can regulate the physiological metabolism of the insect as well as its developmental rate.These findings can facilitate future uses of PTP1B and PTK inhibitors in controlling insect development to help control pest populations.

关 键 词：PTP1B PTK INHIBITOR MAPK pathway Oedaleus decorus asiaticus development 

分 类 号：S433[农业科学—农业昆虫与害虫防治]