复合酶酶解法制备中华草龟皮胶原蛋白肽的工艺优化  

Optimization of Preparation Technology of Chinemys reevesii Skin Collagen Peptide by Compound Enzymes Enzymatic Hydrolysis Method

作　　者：虞丹丹 魏文志[2] 关利 陈文卓 曹晖[1] YU Dan-dan;WEI Wen-zhi;GUAN Li;CHEN Wen-zhuo;CAO Hui(College of Tourism and Culinary Science,Yangzhou University,Yangzhou 225127,China;College of Animal Science and Technology,Yangzhou University,Yangzhou 225001,China)

机构地区：[1]扬州大学旅游烹饪学院,江苏扬州225127 [2]扬州大学动物科学与技术学院,江苏扬州225001

出　　处：《中国调味品》2024年第8期28-33,共6页China Condiment

基　　金：苏北科技专项(2021022)。

摘　　要：该研究探讨了不同种类蛋白酶对中华草龟皮胶原蛋白酶解液的水解度和DPPH自由基清除率的影响,并进一步研究了复合蛋白酶对胶原蛋白的酶解效果。此外,通过正交试验优化了复合酶酶解条件。结果表明,由胰蛋白酶和碱性蛋白酶按1∶1组成的复合酶对龟皮胶原蛋白进行酶解,可显著提高酶解液的水解度和DPPH自由基的清除率(P<0.05);优化的复合酶酶解工艺为复合酶添加量5000 U/g、酶解pH值7.5、酶解温度50℃、酶解时间3 h,在该酶解工艺条件下,水解度达(51.19±2.45)%,DPPH自由基清除率达(51.78±2.21)%。影响复合酶酶解效果的主次顺序为酶添加量>酶解pH值>酶解时间>酶解温度。In this study,the effects of different types of proteases on the hydrolysis degree and DPPH free radical scavenging rate of Chinemys reevesii skin collagen enzymatic hydrolysate are studied,and the enzymatic hydrolysis effect of compound proteases on collagen is further studied.In addition,the enzymatic hydrolysis conditions of compound enzymes are optimized by orthogonal test.The results show that the hydrolysis degree and DPPH free radical scavenging rate of enzymatic hydrolysate are significantly improved(P<0.05)when Chinemys reevesii skin collagen is hydrolyzed by the compound enzymes composed of trypsin and alkaline protease at 1∶1.The optimized compound enzymatic hydrolysis process is as follows:the addition amount of compound enzymes is 5000 U/g,enzymatic hydrolysis pH value is 7.5,enzymatic hydrolysis temperature is 50℃,and enzymatic hydrolysis time is 3 h.Under such enzymatic hydrolysis process conditions,the hydrolysis degree reaches(51.19±2.45)%,and the DPPH free radical scavenging rate reaches(51.78±2.21)%.The primary and secondary order that affects the compound enzymatic hydrolysis effect is enzyme addition amount>enzymatic hydrolysis pH value>enzymatic hydrolysis time>enzymatic hydrolysis temperature.

关 键 词：中华草龟 胶原蛋白肽 复合酶酶解 胰蛋白酶 碱性蛋白酶 

分 类 号：TS201.25[轻工技术与工程—食品科学]