柑橘属植物果皮抑制α-糖苷酶活性的化学计量学分析  

Stoichiometry Analysis on Inhibition ofα-Glycosidase Activity of Citrus Peel

作　　者：闫平[1] 张彦[1] 张华[1] 张寒[1] 郑梦迪 柴希艳 YAN Ping;ZHANG Yan;ZHANG Hua;ZHANG Han;ZHENG Mengdi;CHAI Xiyan(Xi’an Medical University,Xi’an 710021,China;Xi’an Yurun Baide Health Management Co.,Ltd.,Xi’an 710065,China)

机构地区：[1]西安医学院,陕西西安710021 [2]西安雨润百德健康管理有限公司,陕西西安710065

出　　处：《食品科技》2024年第6期239-246,共8页Food Science and Technology

基　　金：陕西省自然科学基础研究项目(2021JQ-782);西安市科技局农业技术研发项目(21NYYF062);西安医学院2021年青年科研基金项目(2021QN23);西安医学院教育教学改革研究项目(2022JG-29)。

摘　　要：目的:研究柑橘属植物果皮对α-糖苷酶抑制活性。方法:高效液相色谱法测定13批柑橘属植物果皮中7种黄酮类成分的含量,并构建指纹图谱。运用聚类分析、主成分分析和偏最小二乘法-判别分析等化学计量学方法,对柑橘属植物果皮与酶抑制作用进行谱效分析。结果:13批柑橘属植物果皮均有较好的α-糖苷酶抑制作用,检测出柚皮甙、橙皮苷、新橙皮苷、橙皮素、甜橙黄酮、川陈皮素、橘皮素等黄酮类成分。化学计量学结果显示,柑橘属植物果皮中橙皮素是主要的α-糖苷酶抑制活性成分。结论:柑橘属植物果皮可应用于开发降血糖的大健康产品中。Objective:To incestigate the inhibitory activity of Citrus peel againstα-glycosidase.Methods:High-performance liquid chromatography(HPLC)was used to determine the content of seven flavonoid components in 13 batches of Citrus peel,establishing a fingerprint.Chemometric methods including cluster analysis,principle component analysis,partial least squares discriminant analysis(PLS-DA),were employed to analyze the spectrum effect relationship between Citrus peel and enzyme inhibition.Results:All 13 batches of Citrus peel had betterα-glycosidase inhibition and contained flavonoid components such as naringin,hesperidin,neohesperidin,hespididin,sweet orange flavone,nobiletin and hesperetin.The stoichiometric results showed that hesperetin was the main component ofα-glycosidase inhibitory activity in Citrus peel.Conclusion:Citrus peel from Citrus plants can be used in the development of health products for hypoglycemia.

关 键 词：柑橘属植物果皮 降糖活性 化学计量学 

分 类 号：TS201.2[轻工技术与工程—食品科学]