超高效液相色谱-串联质谱法测定食品基质中21种食品添加剂  

Determination of 21 Food Additives in Food Matrices by Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：赵超群 梁晶晶 赵竟凯 陶瑞 刘柱 ZHAO Chaoqun;LIANG Jingjing;ZHAO Jingkai;TAO Rui;LIU Zhu(Zhejiang Institute for Food and Drug Control,Key Laboratory of Quality and Safety of Functional Food for State Market Regulation,Key Laboratory of Zhejiang Marketing Bureau(Key Laboratory of Health Food Quality and Safety),Hangzhou 310052,China)

机构地区：[1]浙江省食品药品检验研究院,国家市场监管重点实验室(功能食品质量与安全),浙江省市场监督管理局重点实验室(保健食品质量安全重点实验室),浙江杭州310052

出　　处：《食品科技》2024年第6期342-349,共8页Food Science and Technology

基　　金：浙江省市场监督管理局雏鹰计划核心项目(CY2022227);浙江省药品监督管理局项目(2023004)。

摘　　要：文章建立了超高效液相色谱-三重四级杆串联质谱法同时测定几种食品基质中21种食品添加剂的方法。样品经纯水及乙腈-甲醇-水溶液(V:V:V,1:1:1)振荡和超声提取后,取上清液,用超高效液相色谱-三重四级杆串联质谱在多反应监测下负离子模式分析检测。采用Waters Acquity UPLC HSS T_(3)柱(2.1 mm×100 mm,1.8μm)作为分析柱,梯度洗脱,外标法定量。结果显示:21种添加剂在质量浓度为4.9~771.2 ng/mL范围内线性关系良好,线性相关系数均大于等于0.9903,方法检出限为0.04~0.14 mg/kg,定量限为0.10~0.40 mg/kg;分别向空白样品中添加浓度为2倍、5倍、10倍定量限浓度的21种添加剂标准品,其平均加标回收率为69.2%~118.0%,相对标准偏差为0.59%~10.15%,仪器精密度相对标准偏差为0.68%~4.61%。建立的方法简单、快速,能广泛应用于不同食品基质中多种添加剂的分析检测。A method for simultaneous determination of 21 food additives in several food matrices by ultra high performance liquid chromatography-triple quadrupole tandem mass spectrometry was established.Using pure water and acetonitrile-methanol-water solution(V:V:V,1:1:1)for oscillation and ultrasonic to extracted target compounds in the samples.Ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry was used for analysis and detection in negative ion mode under multi-reaction monitoring(MRM).The separation and analysis were performed on a Waters Acquity UPLC HSS T_(3)(2.1 mm×100 mm,1.8μm),gradient elution,quantitative with external standard method.The results showed that within the range of 4.9–771.2 ng/mL,each target compound had a good linear relationship,and all the correlation coefficient were greater than or equal to 0.9903.The detection limit of the method were 0.04–0.14 mg/kg,and the quantitative limit of the method were 0.10–0.40 mg/kg.The average recoveries of 21 food additives were 69.2%–118.0%when spiked into blank samples at concentrations of 2,5,10 times the limit of quantification(LOQ)concentration,the relative standard deviation were 0.59%–10.15%,and the instrument precision relative standard deviation(RSD)were 0.68%–4.61%.The established method is simple and fast,and can be widely applied to the analysis and detected various additives in different food matrices.

关 键 词：添加剂 食品基质 超高效液相色谱-三重四级杆串联质谱 检测 

分 类 号：TS207.5[轻工技术与工程—食品科学]