超声波辅助双水相萃取鼠曲草总黄酮及抗氧化活性研究  

Study on the Extraction of Total Flavonoids From Pesudognaphalium affine(D.DON)Anderb.and Their Antioxidant Properties by Ultrasonic-assisted Two-phase Aqueous System

作　　者：李燕 曾颖 曾淑欣 庄玲萍 LI Yan;ZENG Ying;ZENG Shuxin;ZHUANG Lingping(Xiamen Medical College,Xiamen 361021,China)

机构地区：[1]厦门医学院,福建厦门361021

出　　处：《特产研究》2024年第4期128-136,142,共10页Special Wild Economic Animal and Plant Research

基　　金：厦门医学院大学生创新创业训练计划资助项目(202312631056)。

摘　　要：本研究以鼠曲草为原料,鼠曲草总黄酮得率为指标,探究超声波辅助双水相萃取鼠曲草总黄酮的最佳工艺路线。利用523双水相试验法,以鼠曲草总黄酮得率为指标,探究双水相最佳组成体系。结果表明,其最佳体系组成为质量分数为16%的磷酸氢二钾,质量分数为30%的乙醇,与乙醇和热水浸提两类传统提取法相比,双水相法具有更好的提取效果,提取率提升了1~2倍。以鼠曲草黄酮得率为响应指标,通过响应面优化超声波辅助双水相萃取体系,得到最优超声波条件参数为超声功率420W、提取时间23 min,超声温度50℃,与常规室温双水相萃取比,其提取率提升了34%左右。测定总抗氧化能力试验结果表明,超声波辅助双水相的黄酮提取物总抗氧化能力约为水提物和乙醇提取物的2倍,乙醇提取物的1.8倍。综上所述,本试验优化构建的超声波辅助双水相萃取法对鼠曲草黄酮具有更高的提取效率,同时能使其提取物保有更强的抗氧化活性。In this experiment,the best technological route of the extraction of total flavonoids by ultrasound-assisted aqueous two-phase from Pesudognaphaliumaffine(D.DON)Anderb.was explored by using Pesudognaphaliumaffine(D.DON)Anderb.as rawmaterial and the yield of total flavonoids from Pesudognaphalium affine(D.DON)Anderb.as an index.Using 523 two-water phase test method,the optimumcomposition systemof two-water phase was studied based on the yield of total flavonoids of Pesudognaphalium affine(D.DON)Anderb.The results showed that the optimal system composition was 16%dipotassium hydrogen phosphate and 30%ethanol.Compared with the traditional extraction methods of ethanol and hot water extraction,the two-aqueous phase method had better extraction effect,and the extraction rate was increased by 1 to 2 times.Taking the yield of Pesudognaphaliumaffine(D.DON)Anderb.as the response index,the optimal ultrasonic process parameters were optimized by response surface optimization,including ultrasonic power 420 W,extraction time 23 min,and ultrasonic temperature 50℃.Compared with the conventional aqueous two-phase extraction at roomtemperature,the extraction yield was increased by 34%.The results of the total antioxidant capacity test highlighted that the total antioxidant capacity of the flavonoid extract of the ultrasonic assisted aqueous phase was about 2 times that of the water extract and 1.8 times that of the ethanol extract.In conclusion,the ultrasound-assisted aqueous two-phase extraction method optimized in this study has a higher extraction efficiency for the flavonoids of Pesudognaphalium affine(D.DON)Anderb.,and at the same time,the extract can maintain a stronger antioxidant activity.

关 键 词：鼠曲草 超声波 双水相 总黄酮 抗氧化活性 

分 类 号：TQ28[化学工程—有机化工]