金针菇基医用复合敷料的制备与研究  

Preparation and study of Flammulina velutipes-basedmedical composite dressing

作　　者：王应雪 谭文丽 季巧 杨凡 文馨可 袁久刚 WANG Yingxue;TAN Wenli;JI Qiao;YANG Fan;WEN Xinke;YUAN Jiugang(College of Textile Science and Engineering,Jiangnan University,Wuxi 214122,China;Jiangsu Textile Union Inspection Technology Service Limited Company,Suzhou 215100,China)

机构地区：[1]江南大学纺织科学与工程学院,无锡214122 [2]江苏中纺联检验技术服务有限公司,苏州215100

出　　处：《生物学杂志》2024年第4期37-42,共6页Journal of Biology

基　　金：国家自然科学基金项目(31771039)。

摘　　要：以多糖提取后的富含几丁质和葡聚糖的金针菇样品为原料,通过冻干法制备金针菇基医用复合敷料。分别采用红外光谱、扫描电镜对材料的形貌及结构进行表征,并测试其抗菌性能、力学性能、吸水保水性能、水蒸气透过率、止血性能、降解性能以及细胞毒性等。结果表明:金针菇样品具有较好的抑菌性能,大肠杆菌抑菌率可达98.83%,金黄色葡萄球菌抑菌率约为88.78%;金针菇基复合敷料的物理性能良好,手感柔软,拉伸应力可达15.20 MPa,断裂伸长率84.11%,吸水保水性优良,水蒸气透过率可达2500 g/(m^(2)·d),体外凝血指数最佳为0.32,而且该敷料无毒无害,丢弃后35 d的土壤降解率即可达到79.79%。结果表明,金针菇基复合敷料具有较好的医用潜力,可用于伤口敷料和组织工程领域。研究对促进真菌多糖提取副产物的利用,提升金针菇附加值具有重要意义。New Flammulina velutipes-based composite material was prepared by freeze-drying method with Flammulina velutipes rich in chitin and dextran as raw material after extraction of polysaccharides.The Flammulina velutipes-based composite was analyzed by infrared spectrum and scanning electron microscope.The samples characteristics such as anti-microbial properties,mechanical properties,swelling property,water vapor permeability,coagulation performance in vitro,degradability and cytotoxicity were assayed.Results showed that Flammulina velutipes samples had excellent antibacterial properties,and the bacteriostatic rates of Escherichia coli and Staphylococcus aureus were 98.51%and 88.18%respectively.Flammulina velutipes-based composite dressing had excellent physical properties and high softness,and had excellent water absorption and water retention.Its breaking strength was 15.20 MPa and elongation was 84.11%.Its water vapor permeability number reached 2500 g/(m^(2)·d),the coagulation index in vitro was 0.3.Moreover,the dressing was non-toxic and harmless,and the soil degradation rate could reach 79.79%after 35 days of discarding.This study demonstrated that Flammulina velutipes-based composite dressing had excellent medical potential and could be used in wound dressing and tissue engineering.This study was of great significance to promote the utilization of by-products of fungal polysaccharide extraction and enhance the added value of Flammulina velutipes.

关 键 词：真菌复合材料 医用敷料 金针菇 几丁质 明胶 

分 类 号：Q949.32[生物学—植物学] TS959.9[轻工技术与工程]