Fanlian Huazhuo Formula alleviates high-fat diet-induced nonalcoholic fatty liver disease by modulating autophagy and lipid synthesis signaling pathway  

作　　者：Meng-Yuan Niu Geng-Ting Dong Yi Li Qing Luo Liu Cao Xi-Min Wang Qi-Wen Wang Yi-Ting Wang Zhe Zhang Xi-Wen Zhong Wei-Bo Dai Le-Yu Li 

机构地区：[1]Pharmacology Laboratory,Zhongshan Hospital of Traditional Chinese Medicine Affiliated to Guangzhou University of Traditional Chinese Medicine,Zhongshan 528400,Guangdong Province,China [2]Department of Endocrinology,Zhongshan Hospital of Traditional Chinese Medicine Affiliated to Guangzhou University of Traditional Chinese Medicine,Zhongshan 528400,Guangdong Province,China

出　　处：《World Journal of Gastroenterology》2024年第30期3584-3608,共25页世界胃肠病学杂志（英文版）

基　　金：Supported by Basic and Applied Basic Research Found of Guangdong Province,No.2022A1515011307。

摘　　要：BACKGROUND Fanlian Huazhuo Formula(FLHZF)has the functions of invigorating spleen and resolving phlegm,clearing heat and purging turbidity.It has been identified to have therapeutic effects on type 2 diabetes mellitus(T2DM)in clinical application.Non-alcoholic fatty liver disease(NAFLD)is frequently diagnosed in patients with T2DM.However,the therapeutic potential of FLHZF on NAFLD and the underlying mechanisms need further investigation.AIM To elucidate the effects of FLHZF on NAFLD and explore the underlying hepatoprotective mechanisms in vivo and in vitro.METHODS HepG2 cells were treated with free fatty acid for 24 hours to induce lipid accumulation cell model.Subsequently,experiments were conducted with the different concentrations of freeze-dried powder of FLHZF for 24 hours.C57BL/6 mice were fed a high-fat diet for 8-week to establish a mouse model of NAFLD,and then treated with the different concentrations of FLHZF for 10 weeks.RESULTS FLHZF had therapeutic potential against lipid accumulation and abnormal changes in biochemical indicators in vivo and in vitro.Further experiments verified that FLHZF alleviated abnormal lipid metabolism might by reducing oxidative stress,regulating the AMPKα/SREBP-1C signaling pathway,activating autophagy,and inhibiting hepatocyte apoptosis.CONCLUSION FLHZF alleviates abnormal lipid metabolism in NAFLD models by regulating reactive oxygen species,autophagy,apoptosis,and lipid synthesis signaling pathways,indicating its potential for clinical application in NAFLD.

关 键 词：Fanlian Huazhuo Formula Nonalcoholic fatty liver disease AUTOPHAGY Apoptosis AMPKα/SREBP-1C signal pathway Oxidative stress 

分 类 号：R575[医药卫生—消化系统]