Metabolomic-based analysis reveals bile acid-mediated ovarian failure induced by low temperature in zebrafish  

作　　者：Wen-Hao Li Zhi-Qiang Li Meng-Di Bu Jia-Zhen Li Liang-Biao Chen 

机构地区：[1]Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306,China [2]Laboratory for Marine Biology and Biotechnology,Qingdao National Laboratory for Marine Science and Technology,Qingdao,Shandong 266237,China [3]International Research Center for Marine Biosciences,Ministry of Science and Technology,College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306,China

出　　处：《Zoological Research》2024年第4期791-804,共14页动物学研究（英文）

基　　金：National Natural Science Foundation of China(32130109);Open Project Fund from Laboratory for Marine Biology and Biotechnology,Qingdao National Laboratory for Marine Science(OF2019NO01)。

摘　　要：As ectotherms, fish are highly sensitive to temperature fluctuations, which can profoundly impact their reproductive cycles. In this study, we investigated the fertility and histological characteristics of zebrafish(Danio rerio) ovaries exposed to a temperature gradient ranging from the thermopreferendum temperature of the species,27℃, to lower temperatures of 22℃, 20℃, and 13℃ over a period of two weeks. Comparative metabolomic(six biological replicates for each temperature) and transcriptomic(four biological replicates for each temperature) analyses were conducted under the four temperature conditions. Results indicated that lower temperatures inhibited oocyte development and differential metabolites were involved in steroid hormone production,antioxidant function, and lipid and protein catabolism.Disrupted reproductive hormones, increased proteolysis,and lipid degradation significantly impeded oocyte development and egg maturation. Notably, a significant increase in bile acid content was noted in the ovaries of the cold-treated fish, indicating that bile acids play a critical role in ovarian failure. Overall, these findings provide valuable insights into the mechanisms governing the reproductive response of fish to cold stress.

关 键 词：old stress Ovarian failure METABOLOME ZEBRAFISH 

分 类 号：Q958[生物学—动物学]