广叶绣球菌多糖-白藜芦醇纳米粒子的制备、表征及其体外抑制α-葡萄糖苷酶活性  

Preparation,characterization and inhibition effects onα-glucosidase of Sparassis latifolia polysaccharides-resveratrol nanoparticles

作　　者：毕书瑜 崔芳铭 侯江燕 董鹏飞 曹谨玲 程艳芬[1,2] 程菲儿 云少君[1,2] 冯翠萍 BI Shuyu;CUI Fangming;HOU Jiangyan;DONG Pengfei;CAO Jinling;CHENG Yanfen;CHENG Fei’er;YUN Shaojun;FENG Cuiping(College of Food Science and Engineering,Shanxi Agricultural University,Jinzhong 030801,Shanxi,China;Shanxi Key Laboratory of Edible Fungi for Loess Plateau,Jinzhong 030801 Shanxi,China)

机构地区：[1]山西农业大学食品科学与工程学院,山西晋中030801 [2]黄土高原食用菌山西省重点实验室,山西晋中030801

出　　处：《菌物学报》2024年第7期90-100,共11页Mycosystema

基　　金：山西省现代农业产业技术体系建设项目(2023CYJSTX09);山西省基础研究计划(202103021224126);食用菌山西省科技创新重点团队(201805D131009)。

摘　　要：本研究采用反溶剂沉淀法制备广叶绣球菌多糖(Sparassis latifolia polysaccharides,SLPs)-白藜芦醇(resveratrol,Res)纳米粒子(SLPs-Res NPs),对其表征后分析其体外抑制α-葡萄糖苷酶活性。试验采用水提醇沉法提取SLPs并经HZ830大孔树脂脱色除杂后,以包封率、载药量为指标优化纳米粒子制备工艺,对其表征后分析其缓释特性及对α-葡萄糖苷酶的抑制作用。结果表明,当Res与SLPs的质量比为1:32时,SLPs-Res NPs的包封率最高,为(53.73±0.76)%,载药量为(1.68±0.01)%。扫描电镜显示SLPs-Res NPs表面较为粗糙。SLPs-Res NPs平均粒径为(223.51±3.02)nm,PDI值为0.51±0.04,Zeta电位为(–21.6±1.1)mV,紫外/可见及红外光谱显示SLPs与Res发生了相互作用。SLPs-Res NPs的光热稳定性均强于游离白藜芦醇,其在模拟胃液中消化2 h后Res释放率为(9.13±0.54)%,继续在模拟肠液中消化3 h后Res释放率为(31.71±0.05)%,显著低于游离Res(P<0.05)。SLPs-Res NPs对α-葡萄糖苷酶的抑制活性显著高于Res和SLPs(P<0.05)。本研究制备得到SLPs-Res NPs,其具有较好的光热稳定性、缓释特性及抑制α-葡萄糖苷酶作用,可为SLPs和Res的进一步开发利用提供一定的理论依据。An antisolvent precipitation method was used to prepare Sparassis latifolia polysaccharides(SLPs)-resveratrol(Res)nanoparticles(SLPs-Res NPs).After characterization,their inhibition activity to α-glucosidase was analyzed in vitro.The Sparassis latifolia polysaccharides were extracted by water extraction and alcohol precipitation method followed by purification with HZ830 macroporous resin,and then the preparation process of SLPs-Res NPs was optimized by using encapsulation efficiency and drug loading as evaluation indicators.After characterization,the sustained-release property and their inhibition effect onα-glucosidase were determined.Results revealed that when the mass ratio of Res to SLPs was 1:32,the encapsulation efficiency of SLPs-Res NPs was(53.73±0.76)%,showing the highest,and the drug loading was(1.68±0.01)%.The scanning electron microscopy showed that the surface of SLPs-Res NPs was relatively rough.The average particle size of SLPs-Res NPs was(223.51±3.02)nm.The PDI value and Zeta potential was(0.51±0.04)and(-21.6±1.1)mV,respectively.The UV/vis and Fourier transform infrared spectra exhibited that SLPs interacted with Res.The photothermal stability of SLPs-Res NPs was stronger than that of free Res.The release rate of Res from SLPs-Res NPs after 2 h of digestion in simulated gastric juice was(9.13±0.54)%,and after 3 h of digestion in simulated intestinal juice,the release rate of Res was(31.71±0.05)%,being significantly lower than that of free Res(P<0.05).The inhibitory activity of SLPs-Res NPs toα-glucosidase was significantly higher than that of Res and SLPs(P<0.05).The SLPs-Res NPs prepared in this study have good photothermal stability,sustained-release property,and inhibitory effect onα-glucosidase and are worthy of further development and utilization.

关 键 词：广叶绣球菌多糖 白藜芦醇 纳米粒子 表征 Α-葡萄糖苷酶 

分 类 号：TS201.2[轻工技术与工程—食品科学]