蛹虫草β-葡聚糖提取工艺优化及抗氧化活性  

Optimization ofβ-Glucan Extraction Process and Antioxidant Activity of Cordyceps militaris

作　　者：林烨欣 陈晖 王雅茹 余蕾[3] 张怡评 洪专 LIN Yexin;CHEN Hui;WANG Yaru;YU Lei;ZHANG Yiping;HONG Zhuan(College of Advanced Manufacturing,Fuzhou University,Quanzhou 362200,Fujian,China;The Engineering Innovation Center for the Development and Utilization of Marine Bioresources,The Ministry of Natural Resources,Xiamen 361005,Fujian,China;Xiamen Ocean Vocational College,Xiamen 361022,Fujian,China;College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350001,Fujian,China)

机构地区：[1]福州大学先进制造学院,福建泉州362200 [2]自然资源部第三海洋研究所海洋生物资源开发利用工程技术创新中心,福建厦门361005 [3]厦门海洋职业技术学院,福建厦门361022 [4]福建农林大学食品科学学院,福建福州350001

出　　处：《食品研究与开发》2024年第15期124-131,163,共9页Food Research and Development

基　　金：厦门市科技计划项目(3502Z20221036、3502ZCQXT2021010)。

摘　　要：以β-葡聚糖得率得率为主要评价指标,通过单因素和响应面试验优化蛹虫草β-葡聚糖超声辅助水提工艺,采用红外扫描分析提取物的化学键及官能团信息,测定其中各组分含量及其体外抗氧化活性。结果表明,蛹虫草β-葡聚糖最优提取工艺为超声时间1 h、料液比1∶31(g/mL)、水浴温度90℃。最优条件下提取得率为(1.94±0.28)%,与预测值1.95%一致。经过纯化试验得出α-葡聚糖去除最优条件为α-淀粉酶添加量2%、孵育时间60 min、温度50℃,经过纯化处理后β-葡聚糖纯度由19.82%提高至33.93%;红外扫描结果表明,蛹虫草β-葡聚糖具有典型的多糖结构,并含有硫酸根基团。蛹虫草β-葡聚糖对DPPH自由基、ABTS+自由基和羟基自由基的清除能力与浓度呈正相关(2.00~10.00 mg/mL),IC_(50)值分别为1.17、2.17、4.35 mg/mL,清除率在浓度为8.34、9.88、9.97 mg/mL时,可达100%。综上,优化后的蛹虫草β-葡聚糖超声辅助水提工艺稳定可行,提取获得的β-葡聚糖具有较好的抗氧化活性。The ultrasound-assisted extraction process ofβ-glucan from Cordyceps militaris was optimized by single factor experiment and response surface methodology withβ-glucan extraction yield as the main evaluation index.The chemical bonds and functional groups of the extracts were analyzed by infrared scanning,and the content of each component and its antioxidant activity in vitro were determined.The results showed that the optimal extraction process ofβ-glucan from C.militaris was ultrasonication time of 1 h,material-liquid ratio of 1∶31(g/mL),and temperature of 90°C.The extraction rate under the optimal conditions was(1.94±0.28)%,which was consistent with the predicted value of 1.95%.The purification test revealed that the optimal conditions forα-glucan removal wereα-amylase addition of 2%,incubation time of 60 min,and temperature of 50℃.The purity ofβ-glucan increased from 19.82%to 33.93%after the purification treatment.The result of infrared scanning showed that C.militarisβ-glucan had a typical polysaccharide structure with sulfate moieties.The scavenging abilities of C.militarisβ-glucan on DPPH radicals,ABTS+radicals,and hydroxyl radicals were positively correlated with the concentration(2.00-10.00 mg/mL),with IC_(50)values of 1.17,2.17 mg/mL and4.35 mg/mL respectively.The scavenging rate was 100%at the concentration of 8.34,9.88 and 9.97 mg/mL.In conclusion,the optimized ultrasound-assisted extraction process ofβ-glucan from C.militaris was stable and feasible,and the antioxidant activity of theβ-glucan extracted was good.

关 键 词：蛹虫草β-葡聚糖 超声辅助水提法 红外分析 抗氧化 β-葡聚糖检测方法 

分 类 号：TS201.2[轻工技术与工程—食品科学]