QuEChERS-高效液相色谱法测定食品中溴硝醇残留量  

QuEChERS-high performance liquid chromatography for determination of residue bronopol in foods

作　　者：彭晓俊 易碧华 梁伟华 秦汉 PENG Xiao-Jun;YI Bi-Hua;LIANG Wei-Hua;QIN Han(Food Laboratory of Jiangmen Customs District Technology Center,Jiangmen 529100,China)

机构地区：[1]江门海关技术中心食品实验室,江门529100

出　　处：《食品安全质量检测学报》2024年第13期234-240,共7页Journal of Food Safety and Quality

基　　金：海关总署科技计划项目(2023HK133)。

摘　　要：目的 建立QuEChERS-高效液相色谱法测定食品中溴硝醇残留量的分析方法 。方法 前处理采用QuEChERS方法 ,样品粉碎后用甲醇提取,改性多壁碳纳米管净化, Agilent ZORBAX Eclipse XDB-C18色谱柱(250 mm×4.6 mm, 5μm)对净化液进行分离,以甲醇-乙腈(含0.1%磷酸)体系作为流动相, 210 nm波长下检测分析。结果 在优化实验条件下,溴硝醇在0.02~10.00μg/mL范围内具有良好的线性关系,相关系数大于0.9999,检出限(S/N=3)为0.050 mg/kg,加标回收率为95.3%~104.0%,相对标准偏差为4.2%~5.3%,实际样品检测中均未检出溴硝醇。结论 该方法 简单、快速、准确、灵敏度高,可应用于粮食、水果和蔬菜中溴硝醇残留的快速检测。Objective To establish a method for determination of bronopol residue in foods by QuEChERS-high performance liquid chromatography.Methods The QuEChERS method was adopted for pretreatment.The sample was extracted using methanol after crushing,and purificated by MWNTs,then separated by Agilent ZORBAX Eclipse XDB-C18 column(250 mm×4.6 mm,5μm)using a methanol acetonitrile(containing 0.1%phosphoric acid)system as mobile phase,and detected at 210 nm.Results Under the optimized experimental conditions,there was a good linear relationship in the range of 0.02-10.00μg/mL,and the correlation coefficient was greater than 0.9999.The limit of detection(S/N=3)was 0.050 mg/kg,the recovery was 95.3%-104.0%,and the relative standard deviation was 4.2%-5.3%.No bromonitrosol was detected in the actual samples.Conclusion The method is simple,rapid,accurate and sensitive,and can be applied to the rapid detection of bromonitol residues in grain,fruits and vegetables.

关 键 词：高效液相色谱法 QUECHERS 改性多壁碳纳米管 溴硝醇 

分 类 号：TS207.5[轻工技术与工程—食品科学] O657.72[轻工技术与工程—食品科学与工程]