响应面法优化桔梗总皂苷微波提取工艺及其抗炎活性分析  

Optimization of microwave extraction process of Platycodon grandiflorus total saponins by response surface method and analysis of its anti-inflammatory activity

作　　者：孙萍 徐慧 王珊珊[1] 李泽润 聂玉朋 刘建军[1] SUN Ping;XU Hui;WANG Shan-shan;LI Ze-run;NIE Yu-peng;LIU Jian-jun(Shandong Food Ferment Industry Research and Design Institute,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250013,Shandong,China)

机构地区：[1]齐鲁工业大学(山东省科学院)山东省食品发酵工业研究设计院,山东济南250013

出　　处：《粮食与油脂》2024年第8期94-99,共6页Cereals & Oils

基　　金：齐鲁工业大学(山东省科学院)科教与产业融合重大创新试点项目(2022JBZ01-08);山东省科技厅山东省自然科学基金面上项目(ZR2020MC202)。

摘　　要：采用微波辅助提取桔梗总皂苷。以总皂苷得率为指标,在单因素试验的基础上,通过响应面法优化提取工艺,并研究桔梗总皂苷的抗炎活性。结果显示:最佳提取条件为微波功率210 W、微波时间3.5 min、固液比1∶17.5(g/mL)、乙醇体积分数80%,在此条件下桔梗总皂苷得率为5.5%。抗炎活性试验结果表明,桔梗总皂苷含有抑制炎症因子,具有较好的抗炎活性。The total saponins of Platycodon grandiflorus were extracted by microwave-assisted extraction.Taking yield of total saponins as an indicator,and the extraction process was optimized by response surface methodology based on single factor experiments,and the anti-inflammatory activity of total saponins of Platycodon grandiflorus was investigated.The results showed that the optimal extraction conditions were microwave power 210 W,microwave time 3.5 min,solid-liquid ratio 1:17.5(g/mL),and volume fraction of ethanol 80%,and the total saponins yield of Platycodon grandiflorus was 5.5% under these conditions.The results of the anti-inflammatory activity test showed that the total saponins of Platycodon grandiflorus contained inhibitory inflammatory factors and had good anti-inflammatory activity.

关 键 词：桔梗 总皂苷 微波提取 抗炎活性 

分 类 号：TS201.1[轻工技术与工程—食品科学]