樟树籽仁油的遗传毒性评价  

Genotoxicity evaluation of Cinnamomum camphora seed kernel oil

作　　者：彭婷[1,2,3] 梁丽军 曾哲灵 张淑虹[1,5] 鄢祥辉 夏佳恒 余平 万冬满 文学方[7,8] PENG Ting;LIANG Lijun;ZENG Zheling;ZHANG Shuhong;YAN Xianghui;XIA Jiaheng;YU Ping;WAN Dongman;WEN Xuefang(State Key Laboratory of Food Science and Resources,Nanchang University,Nanchang 330047,China;Jiangxi Province Key Laboratory of Edible and Medicinal Resources Exploitation,Nanchang 330031,China;School of Food Science and Technology,Nanchang University,Nanchang 330031,China;Pure Circle(Jiangxi)Co.,Ltd.,Ganzhou 341108,Jiangxi,China;School of Chemistry and Chemical Engineering,Nanchang University,Nanchang 330031,China;School of Resources and Environment,Nanchang University,Nanchang 330031,China;Institute of Applied Chemistry,Jiangxi Academy of Sciences,Nanchang 330096,China;Institute of Traditional Chinese Medicine Health Industry,China Academy of Chinese Medical Sciences,Nanchang 330115,China)

机构地区：[1]南昌大学食品科学与资源挖掘全国重点实验室,南昌330047 [2]江西省药食同源植物资源高值化利用重点实验室,南昌330031 [3]南昌大学食品学院,南昌330031 [4]谱赛科(江西)生物技术有限公司,江西赣州341108 [5]南昌大学化学化工学院,南昌330031 [6]南昌大学资源与环境学院,南昌330031 [7]江西省科学院应用化学研究所,南昌330096 [8]中国中医科学院中医药健康产业研究所,南昌330115

出　　处：《中国油脂》2024年第8期75-81,共7页China Oils and Fats

基　　金：国家国际科技合作专项项目(2011DFA32770);国家自然科学基金项目(31701651,32060516);江西省科技支撑计划重大项目(20143ACG70015);南昌大学食品与技术国家重点实验室自由探索课题(SKLF-ZZB-202135,SKLFZZB-201916);江西省重点研发计划项目(20212BBF63035);江西省科学院省级包干制项目(2022YSBG21021);江西省科学院省级科研院基础研究项目(2022YJC2016)。

摘　　要：为评价樟树籽仁油的食用安全性,通过细菌回复突变试验、体外哺乳类细胞TK基因突变试验及哺乳动物红细胞微核试验探究樟树籽仁油的致突变和潜在致癌作用。结果表明:樟树籽仁油对组氨酸缺陷型鼠伤寒沙门氏菌TA1535、TA97、TA98、TA100、TA102均无致突变作用;樟树籽仁油未抑制L5178Y小鼠淋巴瘤细胞的生长,对体外哺乳类细胞TK基因无致突变作用;樟树籽仁油未导致小鼠细胞微核率上升,对哺乳动物红细胞无致突变作用,无遗传毒性。综上,樟树籽仁油无细胞致突变和潜在致癌作用,没有遗传毒性,具有一定的食用安全性。To evaluate the edible safety of Cinnamomum camphora seed kernel oil(CCSKO),the mutagenicity and potential carcinogenic effects of CCSKO were investigated by bacterial reverse mutation tests,in vitro mammalian cell TK gene mutation tests,and mammalian erythrocyte micronucleus tests.The results showed that CCSKO had no mutagenic effect on Salmonella typhimurium strains TA1535,TA97,TA98,TA100 and TA102 with histidine deficiency.CCSKO did not inhibit the growth of L5178Y mouse lymphoma cells,and had no mutagenic effect on TK gene in mammalian cells in vitro.CCSKO did not increase the micronucleus rate of cell in mice,had no mutagenic effect on mammalian erythrocytes and had no genotoxicity.To sum up,CCSKO has no mutagenicity and potential carcinogenic effect,no genotoxicity,and has certain edible safety.

关 键 词：樟树籽仁油 食用安全性 遗传毒性 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程] TS201.6[轻工技术与工程—食品科学与工程]