超声-酶辅助碱法提取黑木耳膳食纤维及对糖尿病小鼠的降血糖作用  

Ultrasound-enzyme-assisted alkali method for extracting dietary fiber from Auricularia auricula and its hypoglycemic effect on diabetic mice

作　　者：杨帆 孙孝帆 李德海 王璐 王楠楠 王鹏 YANG Fan;SUN Xiaofan;LI Dehai;WANG Lu;WANG Nannan;WANG Peng(College of Life Sciences,Northeast Forestry University,Haerbin 150040,Heilongjiang,China;Heilongjiang Key Laboratory of Forest Food Resources Utilization,Haerbin 150040,Heilongjiang,China;Huadi Computer Group Co.,Ltd.,Beijing 100195,China)

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040 [2]黑龙江省森林食品资源利用重点实验室,黑龙江哈尔滨150040 [3]华迪计算机集团有限公司,北京100195

出　　处：《精细化工》2024年第8期1754-1764,1847,共12页Fine Chemicals

基　　金：黑龙江省自然科学基金资助项目(LH2022C007,C2015062);黑龙江省高等教育教学改革项目(SJGY20210021);哈尔滨市科技局创新研究基金项目(2017RAQXJ091)。

摘　　要：以提取多糖后的黑木耳残渣粉末为原料,采用超声-酶辅助碱法提取黑木耳膳食纤维(AADF),通过单因素实验和Box-Behnken设计确定了AADF的最佳提取工艺,进一步通过小鼠体内实验探究了AADF的降血糖功效。结果表明,在料液比(g∶mL)1∶15、超声功率350 W、超声时间50 min、耐高温α-淀粉酶用量5.0%(以黑木耳残渣粉末的质量为基准,下同)的最佳工艺下,AADF提取率为69.18%。28 d经AADF干预糖尿病小鼠饮食期间,模型高剂量组[每天AADF灌胃量为200mg/(kg·bw)]与模型对照组(每天灌胃生理盐水)的糖尿病小鼠相比,空腹血糖值下降13.72%、口服糖耐量降低16.95%,血脂总胆固醇和甘油三酯含量显著降低,高密度脂蛋白胆固醇和血清中谷胱甘肽过氧化物酶的水平升高,肝脏中的过氧化氢酶以及琥珀酸脱氢酶活力得到提高,与模型阳性组[每天二甲双胍灌胃量为100 mg/(kg·bw)]各项生理指标相近;肝脏组织病理学分析也表明,AADF对糖尿病小鼠肝损伤具有一定的修复作用。Taking Auricularia auricula residue powder after polysaccharides extracted as raw materials,Auricularia auricula dietary fiber(AADF)was extracted by ultrasound-enzyme-assisted alkali method,and the optimal extraction process was determined by single-factor experiments and Box-Behnken design.The hypoglycemic effect of AADF was further explored by in vivo experiments in mice.The results showed that the extraction rate of AADF reached 69.18%under the optimal process of material liquid ratio(g∶mL)1∶15,ultrasonic power 350 W,ultrasonic time 50 min,and 5.0%α-amylase with high temperature resistance(based on the mass of Auricularia auricula residue powder,the same below).Data from in vivo experiments of diabetic mice with AADF intervention for 28 d indicated that,in comparison with those of the control group treated with normal saline per day,the fasting blood glucose value of the group treated with high dosage AADF[200 mg/(kg·bw)per day]decreased by 13.72%,oral glucose tolerance decreased by 16.95%,the contents of total cholesterol and triglycerides in blood lipids were significantly reduced,and the levels of high-density lipoprotein cholesterol and glutathione peroxidase in serum were increased.The activity of catalase and succinate dehydrogenase in the liver was improved,which was similar to the physiological indexes of the model-positive group[metformin gavage amount of 100 mg/(kg·bw)per day].Histopathological analysis of the liver also demonstrated that AADF showed a certain repair effect on liver injury in diabetic mice.

关 键 词：黑木耳 膳食纤维 工艺优化 小鼠 降血糖活性 中药现代化技术 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]