RAD51B-AS1 promotes the malignant biological behavior of ovarian cancer through upregulation of RAD51B  

作　　者：Xinyi WEI Conghui WANG Sangsang TANG Qian YANG Zhangjin SHEN Jiawei ZHU Xiaodong CHENG Xinyu WANG Xing XIE Junfen XU Weiguo LU 

机构地区：[1]Women's Reproductive Health Laboratory of Zhejiang Province,Women�s Hospital,Zhejiang University School of Medicine,Hangzhou 310006,China [2]Department of Gynecologic Oncology,Women�s Hospital,Zhejiang University School of Medicine,Hangzhou 310006,China [3]Cancer Center,Zhejiang University,Hangzhou 310058,China [4]Department of Obstetrics and Gynecology,the First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China [5]Zhejiang Provincial Clinical Research Center for Obstetrics and Gynecology,Hangzhou 310006,China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2024年第7期581-593,共13页浙江大学学报（英文版）B辑（生物医学与生物技术）

摘　　要：Long non-coding RNAs(lncRNAs)play an indispensable role in the occurrence and development of ovarian cancer(OC).However,the potential involvement of lncRNAs in the progression of OC is largely unknown.To investigate the detailed roles and mechanisms of RAD51 homolog B-antisense 1(RAD51B-AS1),a novel lncRNA in OC,reverse transcription-quantitative polymerase chain reaction(RT-qPCR)was performed to verify the expression of RAD51B-AS1.Cellular proliferation,metastasis,and apoptosis were detected using the cell counting kit-8(CCK-8),colony-formation,transwell,and flow cytometry assays.Mouse xenograft models were established for the detection of tumorigenesis.The results revealed that RAD51B-AS1 was significantly upregulated in a highly metastatic human OC cell line and OC tissues.RAD51B-AS1 significantly increased the proliferation and metastasis of OC cells and enhanced their resistance to anoikis.Biogenetics prediction analysis revealed that the only target gene of RAD51B-AS1 was RAD51B.Subsequent gene function experiments revealed that RAD51B exerts the same biological effects as RAD51B-AS1.Rescue experiments demonstrated that the malignant biological behaviors promoted by RAD51B-AS1 overexpression were partially or completely reversed by RAD51B silencing in vitro and in vivo.Thus,RAD51B-AS1 promotes the malignant biological behaviors of OC and activates the protein kinase B(Akt)/B cell lymphoma protein-2(Bcl-2)signaling pathway,and these effects may be associated with the positive regulation of RAD51B expression.RAD51B-AS1 is expected to serve as a novel molecular biomarker for the diagnosis and prediction of poor prognosis in OC,and as a potential therapeutic target for disease management.

关 键 词：Ovarian cancer(OC) Long non-coding RNA(LncRNA) Metastasis ANOIKIS 

分 类 号：R737.31[医药卫生—肿瘤]