开化龙顶绿茶对高脂小鼠肝脂肪变性AMPK信号通路和肠道菌群的影响  

Effect of Kaihua Longding Green Tea on AMPK Signaling Pathway of Hepatic Steatosis and Gut Microbiota Dysbiosis in High-fat Diet Mice

作　　者：张一帆 袁懿炜 徐倩倩 龚明秀 叶江成 赵进 马崭阳 Zhang Yifan;Yuan Yiwei;Xu Qianqian;Gong Mingxiu;Ye Jiangcheng;Zhao Jin;Ma Zhanyang(Institute of Food Nutrition,Quality and Safety,College of Life Sciences,China Jiliang University,Hangzhou 310018;Key Laboratory of Specialty Agri-product Quality and Hazard Controling Technology of Zhejiang Province,Hangzhou 310018)

机构地区：[1]中国计量大学生命科学学院食品营养与质量安全研究所,杭州310018 [2]特色农产品品质与危害物控制技术浙江省重点实验室,杭州310018

出　　处：《中国食品学报》2024年第7期157-171,共15页Journal of Chinese Institute Of Food Science and Technology

基　　金：衢州市科技攻关竞争性项目(2023k098);浙江省团队科技特派员项目(2020-2024开化县茶产业提升);浙江省重点研发计划项目(2020C02045)。

摘　　要：目的:探究开化龙顶绿茶水提物对高脂饮食小鼠肝脂肪变性AMPK信号通路和肠道菌群紊乱的干预效果。方法:检测开化龙顶绿茶加工环节茶样主要功能成分动态变化趋势。适应性饲养30只小鼠1周后,随机将其分为正常组、高脂组和龙顶绿茶组,各10只,连续口服灌胃8周(正常组和高脂组灌胃生理盐水,龙顶绿茶组灌胃300 mg/kg bw的茶汤水提物),记录并分析小鼠体质量、Lee's指数、血糖水平、血脂、肝组织病理切片、肝组织AMPK信号通路和肠道菌群的变化。结果:在加工过程中,开化龙顶绿茶的主要功能成分均发生一定程度改变。与鲜叶相比,提香中茶多酚、游离氨基酸、咖啡碱和水分的含量分别降低了7.90%,6.20%,0.47%以及71.05 g/100 g,而可溶性糖和水浸出物的含量分别升高了0.01%和0.10%;与高脂组相比,龙顶绿茶组小鼠的体质量为26.67 g,Lee's指数为3.13,血糖曲线下面积为1505.25 mmol·min/L,血清生化指标如总胆固醇(TC)和总甘油三酯(TG)的含量分别为4.23 mmol/L和0.76 mmol/L,均表现出明显差异(P<0.05)。并且,开化龙顶绿茶水提物还能显著降低肝组织AMPK信号通路基因转录和蛋白表达水平(P<0.05);开化龙顶绿茶水提物可显著降低高脂小鼠肠道菌群中厚壁菌门与拟杆菌门的比值(P<0.05),并增加理研菌科、布劳特氏菌属和另枝菌属等有益菌种的相对丰度。结论:开化龙顶绿茶水提物干预高脂饮食小鼠肝脂肪变性AMPK信号通路和肠道菌群紊乱的效果明显。Objective:To explore the effect of Kaihua Longding green tea water extract on intervening AMPK signaling pathway of liver hepatic steatosis and gut microbiota dysbiosis in high-fat diet mice.Methods:Detect the dynamic changes trend of main functional components of samples from each processing procedures in Kaihua Longding green tea.After acclimation feeding for 1 week,30 mice were randomly divided into the following three groups:normal control group(NC),high-fat diet group(HF)and Longding green tea group(LD),and were continuously administrated(NC) and HF were given normal saline,LD was given tea water extract(300 mg/kg bw)for 8 weeks.The changes of body weight,Lee's index,blood glucose levels,serum lipid,pathobiology in liver tissues,AMPK signaling pathway in liver and gut microbiota were measured.Results:During processing procedures,the main functional components of Kaihua Longding green tea undergone certain changes.Compared to the"Fresh leaves"sample,the contents of tea polyphenol,free amino acids,caffeine,and moisture in the"Drying"sample were respectively reduced by 7.90%,6.20%,0.47%,and 71.05 g/100 g,while the contents of soluble sugar and water extract were respectively increased by 0.01% and 0.10%.Compared to the HF group,the LD group exhibited a significant difference(P<0.05)in body weight(26.67 g),Lee's index(3.13),area under the glucose curve(1505.25 mmol·min/L),and serum biochemical indices such as total cholesterol(TC)(4.23 mmol/L),and total triglycerides(TG)(0.76 mmol/L),and also reduced the level of AMPK signaling pathway related genes and proteins of liver tissue in mice(P<0.05).The results of gut microbiota analysis showed that Kaihua Longding green tea water extract decreased the ratio of Firmicutes/Bacteroidetes(F/B),and increased the growth of beneficial bacteria,including Rikenellaceae,Blautia and Alistipes.Conclusion:The effect of Kaihua Longding green tea water extract on intervening AMPK signaling pathway of liver hepatic steatosis and gut microbiota dysbiosis in high-fat diet mice was ef

关 键 词：开化龙顶绿茶 高脂饮食 肝脂肪变性 AMPK信号通路 肠道菌群紊乱 

分 类 号：TS201.4[轻工技术与工程—食品科学] TS272.51[轻工技术与工程—食品科学与工程]