白雪茶水提物对乙醇诱导的小鼠急性酒精性肝损伤的干预作用  

Protective Effect and Mechanism of Aqueous Extract of Thamnolia vermicularis Ach on Ethanol-Induced Acute Alcoholic Liver Injury in Mice

作　　者：韩兴 吕静 王兴妮 秦宇 李莉蓉[1] 黄锐 Han Xing;Lu Jing;Wang Xingni;Qin Yu;Li Lirong;Huang Rui(Faculty of Food Science and Engineering,Kunming University of Science and Technology,Kunming 650500;Zhongken Huashan Mu Dairy Co.,Ltd.,Weinan 714000,Shaanxi)

机构地区：[1]昆明理工大学食品科学与工程学院,昆明650500 [2]中垦华山牧乳业有限公司,陕西渭南714019

出　　处：《中国食品学报》2024年第7期172-181,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：云南省科技厅基础研究计划面上项目(202001AT070032);陕西省科技厅厅市联动渭南现代农业重点项目(2022GD-TSLD-58-1)。

摘　　要：目的:研究白雪茶水提物(TWA)对急性酒精性肝损伤(AALI)小鼠的保护作用。方法:以12 mL/kg剂量的TWA灌胃56%乙醇诱导的AALI模型,评价小鼠体质量、肝脏指数、肝功能、酒精代谢能力、氧化应激水平、炎症因子、关键蛋白表达的变化以及对肠道菌群的影响。结果:TWA显著降低AALI小鼠谷丙转氨酶(ALT)和谷草转氨酶(AST)含量,提高乙醇脱氢酶(ADH)含量,改善肝功能,加快酒精代谢。TWA通过提高血清和肝脏中超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-PX)的活性,降低丙二醛(MDA)含量,降低小鼠机体氧化应激水平。此外,TWA通过抑制肝脏和血清中肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)水平来减轻机体炎症反应;通过增强Nrf2通路功能和抑制NF-κB通路活化来抑制AALI小鼠肝组织炎症及提高机体抗氧化应激作用;下调酒精代谢关键蛋白CYP2E1的表达来降低肝脏的酒精代谢损伤。同时,TWA促进乳杆菌和双歧杆菌的增殖,减少肠球菌、大肠杆菌和消化球菌的数量,从而改善AALI小鼠肠道微生态环境。结论:TWA能够提高小鼠肝功能和酒精代谢,增强机体抗氧化能力,降低体内炎症因子水平,抑制Nrf2/NF-κB通路关键蛋白表达并维持肠道稳态,改善小鼠AALI。Objective:The purpose of the present study was to investigate the protective effect of aqueous extract of Thamnolia vermicularis Ach(TWA)on acute alcoholic liver injury(AALI)and its mechanism.Methods:The AALI model was induced by gavage of 56%alcohol at a dose of 12 mL/kg of the experiment.Its mechanisms were evaluated by weight loss,liver index,liver function,ability to metabolize alcohol,oxidative stress levels,inflammatory factors,changes in key protein expression and the effects on intestinal flora.Results:TWA significantly reduced the levels of alanine aminotransferase(ALT)and aspartate aminotransferase(AST),increased the level of ethanol dehydrogenase(ADH),improved liver function and accelerated ethanol metabolism in mice.TWA also increased the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX)in serum and liver,decreased malondialdehyde(MDA)content,and reduced level of oxidative stress in the mouse caused by ethanol.In addition,TWA reduced the inflammatory response of the body by suppressing tumour necrosis factor-α(TNF-α)and interleukin-6(IL-6)levels in the liver and serum.TWA enhanced Nrf2 pathway function and inhibited NF-κB pathway activation,thereby inhibiting liver inflammation and improving the antioxidant stress effect in mice with acute alcoholic liver injury.Meanwhile,the expression of CYP2E1,a key protein of alcohol metabolism,was down-regulated to accelerate hepatic alcohol metabolism and reduce hepatic alcohol metabolic injury.TWA promoted the proliferation of Lactobacillus and Bifidobacterium,and reduced the colony numbers of Enterococcus,E.coli and Peptococcus,so improved the intestinal environment.Conclusion:TWA could improve liver function and alcohol metabolism,enhance antioxidant capacity,reduce the level of inflammatory factors,inhibit the expression of key proteins of Nrf2/NF-κB pathway and maintain intestinal homeostasis,so improve AALI in mice.

关 键 词：白雪茶水提物 肝损伤 氧化应激 炎症 酒精 

分 类 号：TS272[农业科学—茶叶生产加工] TS201.4[轻工技术与工程—农产品加工及贮藏工程]