Investigating the Impact of Ocean Acidification on Anti-Stress Mechanisms in Sepia esculenta Larvae Based on Transcriptome Profiling  

作　　者：WANG Yongjie LIU Xiumei LV Tingjin WANG Weijun SUN Guohua YANG Jianmin LI Zan 

机构地区：[1]School of Agriculture,Ludong University,Yantai 264025,China [2]College of Life Sciences,Yantai University,Yantai 264005,China [3]Yantai Institute of Marine Economy,Yantai 264003,China

出　　处：《Journal of Ocean University of China》2024年第4期1054-1066,共13页中国海洋大学学报（英文版）

基　　金：funded by the Ministry of Agriculture of the People’s Republic of China (No.CARS-49)。

摘　　要：With the rapid development of oil,energy,power and other industries,CO_(2) emissions rise sharply,which will cause a large amount of CO_(2) in the air be absorbed by the ocean and lead to ocean acidification.The growth and development of organisms can be seriously affected by acidified seawater.Sepia esculenta is a mollusk with high nutritional and economic value and is widely cultured in offshore waters of China.Larvae are the early life forms of the organism and are more vulnerable to changes in the external environment.Too low pH will lead to some adverse reactions in larvae,which will affect metabolism,immune response and other life activities.In this study,we sequenced the transcriptome of S.esculenta subjected to acidified seawater stress and identified 1072differentially expressed genes(DEGs).The detected atypical expression of DEGs substantiates cellular malformation and translocation in S.esculenta under low pH stimulation.Simultaneously,this also substantiates the notable impact of ocean acidification on mollusks.These DEGs were used for functional enrichment analysis of GO and KEGG,and the top twenty items of the biological process classification in GO terms and 11 KEGG signaling pathways were significantly enriched.Finally,the constructed proteinprotein interaction network(PPI)was used to analyze protein-protein interactions,and 12 key DEGs and 3 hub genes were identified.The reliability of 12 genes was verified by quantitative RT-PCR.A comprehensive analysis of the KEGG signaling pathway and PPI revealed that ocean acidification leads to abnormalities in lipid metabolism in S.esculenta larvae,which can lead to cancer development and metastasis,accompanied by some degree of inflammation.The results of the study will help to further investigate the physiological processes of S.esculenta when stimulated by ocean acidification,and provide a reference to cope with the captive breeding of S.esculenta affected by acidification.

关 键 词：Sepia esculenta ocean acidification protein-protein interaction network TRANSCRIPTOME IMMUNITY 

分 类 号：X145[环境科学与工程—环境科学] X174[生物学—动物学] Q958.8