Recent advances in optical techniques for dynamically probing cellular mechanobiology  

作　　者：Fengqi Wang Qin Zhang Mo Yang Bohan Yin Siu Hong Dexter Wong 

机构地区：[1]School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China [2]Laboratory for Marine Drugs and Bioproducts,Qingdao Marine Science and Technology Center,Qingdao 266237,China [3]Department of Biomedical Engineering,The Hong Kong Polytechnic University,Hong Kong 999077,China.

出　　处：《Biomedical Engineering Communications》2024年第3期3-11,共9页生物医学工程通讯

基　　金：the funding from Start-up Fundings of Ocean University of China(862401013154 and 862401013155);Laboratory for Marine Drugs and Bioproducts Qingdao Marine Science and Technology Center(LMDBCXRC202401 and LMDBCXRC202402);Taishan Scholar Youth Expert Program of Shandong Province(tsqn202306102 and tsqn202312105);Shandong Provincial Overseas Excellent Young Scholar Program(2024HWYQ-042 and 2024HWYQ-043)for supporting this work.

摘　　要：Cellular mechanotransduction characterized by the transformation of mechanical stimuli into biochemical signals,represents a pivotal and complex process underpinning a multitude of cellular functionalities.This process is integral to diverse biological phenomena,including embryonic development,cell migration,tissue regeneration,and disease pathology,particularly in the context of cancer metastasis and cardiovascular diseases.Despite the profound biological and clinical significance of mechanotransduction,our understanding of this complex process remains incomplete.The recent development of advanced optical techniques enables in-situ force measurement and subcellular manipulation from the outer cell membrane to the organelles inside a cell.In this review,we delved into the current state-of-the-art techniques utilized to probe cellular mechanobiology,their principles,applications,and limitations.We mainly examined optical methodologies to quantitatively measure the mechanical properties of cells during intracellular transport,cell adhesion,and migration.We provided an introductory overview of various conventional and optical-based techniques for probing cellular mechanics.These techniques have provided into the dynamics of mechanobiology,their potential to unravel mechanistic intricacies and implications for therapeutic intervention.

关 键 词：MECHANOBIOLOGY cell adhesion optical techniques live cell imaging cell fates 

分 类 号：R73[医药卫生—肿瘤]