人参枳椇子口服液的解酒保肝作用研究  

Anti-inebriation and Hepatoprotective Effects of Renshen Zhijuzi Oral Liquid

作　　者：郑彦楠 阮郑石诗 高建平[1] 贾晓薇 丁旭彦 袁满 ZHENG Yannan;RUAN Zhengshishi;GAO Jianping;JIA Xiaowei;DING Xuyan;YUAN Man(School of Pharmacy,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai Tonghan Chuntang Pharmaceutical Co.,Ltd.,Shanghai 200012,China)

机构地区：[1]上海中医药大学中药学院,上海201203 [2]上海童涵春堂药业股份有限公司,上海200012

出　　处：《世界中医药》2024年第15期2253-2259,共7页World Chinese Medicine

基　　金：国家自然科学基金项目(82204723)——益母草总生物碱抑制HIF-1α介导的MCs活化抗过敏性哮喘机制研究。

摘　　要：目的:研究人参枳椇子口服液(GROL)的解酒保肝作用。方法:将50只小鼠随机分空白组、模型组、GROL高剂量组、GROL低剂量组和阳性药联苯双酯对照组,每组10只。灌胃给药4周后用50%乙醇一次灌胃建立小鼠急性醉酒模型,考察GROL对小鼠基本生理活动、小鼠血清及肝组织中相关指标,观察肝组织病理切片,评价其解酒保肝作用。采用乙醇诱导AML12肝细胞损伤模型,检测GROL干预后对乙醇刺激的AML12细胞上清液中谷丙转氨酶(GPT)、谷草转氨酶(GOT)、活性氧(ROS)水平的影响。结果:GROL组小鼠醉酒时间显著延长,睡眠、醒酒时间显著缩短;血清中乙醇浓度、GPT、GOT水平显著降低;肝细胞损伤情况有所改善;GROL组肝组织中超氧化物歧化酶(SOD)活性和谷胱甘肽过氧化物酶(GSH)水平明显提高,丙二醛(MDA)含量明显降低。对体外培养的AML12肝细胞,GROL可以逆转乙醇引起的肝细胞ALT和AST活性的升高以及过度生成的ROS量。结论:GROL具有良好的防醉、解酒和保肝作用,其发挥作用的途径可能与促进体内乙醇代谢、增强内源性抗氧化酶的活性有关。Objective:To investigate the effects of Renshen Zhijuzi Oral Liquid(RZOL)on alcohol detoxification and liver protection.Methods:Fifty mice were randomly divided into a blank group,a model group,a high-dose RZOL group,a low-dose RZOL group,and a positive control group(bifendate),with 10 mice in each group.After 4 weeks of oral gavage,an acute alcohol intoxication model was induced using a single dose of 50%ethanol.The effects of RZOL on basic physiological activities and serum and liver tissue indicators were investigated,and pathological sections of liver tissue were observed.The anti-inebriation and hepatoprotective effects of RZOL were assessed.Additionally,an AML12 liver cell injury model was established with ethanol,and the impact of RZOL on ethanol-stimulated AML12 cell supernatants was measured by examining glutamic-pyruvic transaminase(GPT),glutamic oxalacetic transaminase(GOT),and reactive oxygen species(ROS)levels.Results:Mice treated with RZOL had significantly extended intoxication times,shortened sleep time and time to wake-up,significantly reduced serum ethanol concentration,GPT,and GOT levels,improved liver cell damage,increased superoxide dismutase(SOD)activity and glutathione peroxidase(GSH)levels in liver tissue,and reduced malondialdehyde(MDA)content.In AML12 liver cells cultured in vitro,RZOL reversed ethanol-induced increases in ALT and AST activities and excessive ROS production.Conclusion:RZOL demonstrates effective anti-inebriation,detoxification,and liver protection properties.Its mechanism likely involves enhancing ethanol metabolism and boosting endogenous antioxidant enzyme activity.

关 键 词：人参枳椇子口服液 急性酒精性肝病 乙醇 解酒 保肝 抗氧化 乙醇代谢 

分 类 号：R242[医药卫生—中医临床基础] R322.47[医药卫生—中医学]