A promising approach for quantifying focal stroke modeling and assessing stroke progression:optical resolution photoacoustic microscopy photothrombosis  

作　　者：Xiao Liang Xingping Quan Xiaorui Geng Yujing Huang Yonghua Zhao Lei Xi Zhen Yuan Ping Wang Bin Liu 

机构地区：[1]Faculty of Health Sciences,University of Macao,Macao Special Administrative Region,China [2]Center for Cognitive and Brain Sciences,University of Macao,Macao Special Administrative Region,China [3]Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,Guangdong Province,China [4]State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macao,Taipa,Macao Special Administrative Region,China [5]Department of Emergency,Zhujiang Hospital,Southern Medical University,Guangzhou,Guangdong Province,China

出　　处：《Neural Regeneration Research》2025年第7期2029-2037,共9页中国神经再生研究（英文版）

基　　金：supported by University of Macao,China,Nos.MYRG2022-00054-FHS and MYRG-GRG2023-00038-FHS-UMDF(to ZY);the Macao Science and Technology Development Fund,China,Nos.FDCT0048/2021/AGJ and FDCT0020/2019/AMJ and FDCT 0011/2018/A1(to ZY);Natural Science Foundation of Guangdong Province of China,No.EF017/FHS-YZ/2021/GDSTC(to ZY)。

摘　　要：To investigate the mechanisms underlying the onset and progression of ischemic stroke,some methods have been proposed that can simultaneously monitor and create embolisms in the animal cerebral cortex.However,these methods often require complex systems and the effect of age on cerebral embolism has not been adequately studied,although ischemic stroke is strongly age-related.In this study,we propose an optical-resolution photoacoustic microscopy-based visualized photothrombosis methodology to create and monitor ischemic stroke in mice simultaneously using a 532 nm pulsed laser.We observed the molding process in mice of different ages and presented age-dependent vascular embolism differentiation.Moreover,we integrated optical coherence tomography angiography to investigate age-associated trends in cerebrovascular variability following a stroke.Our imaging data and quantitative analyses underscore the differential cerebrovascular responses to stroke in mice of different ages,thereby highlighting the technique's potential for evaluating cerebrovascular health and unraveling age-related mechanisms involved in ischemic strokes.

关 键 词：AGE-DEPENDENT cerebral cortex ischemic stroke mouse model optical coherence tomography angiography photoacoustic microscopy PHOTOTHROMBOSIS vascular imaging 

分 类 号：R743.3[医药卫生—神经病学与精神病学]