菊粉、复合菊粉膳食纤维对肺纤维化小鼠炎症损伤、氧化应激及肠道菌群的影响  

Effects of Inulin or Compound Inulin Dietary Fiber on Inflammatory Injury,Oxidative Stress,and Intestinal Flora of Mice with Pulmonary Fibrosis

作　　者：蒋鲁杰 衣悦涛[1,3] JIANG Lujie;YI Yuetao(Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,Shandong,China;University of Chinese Academy of Sciences,Beijing 100049,China;Shandong Saline-Alkali Land Modern Agriculture Co.,Ltd.,Dongying 257347,Shandong,China)

机构地区：[1]中国科学院烟台海岸带研究所,山东烟台264003 [2]中国科学院大学,北京100049 [3]山东盐碱地现代农业有限公司,山东东营257347

出　　处：《食品研究与开发》2024年第16期81-89,共9页Food Research and Development

基　　金：黄三角国家农高区科技专项(2022SZX01)。

摘　　要：为探讨菊粉或复合菊粉膳食纤维对肺纤维化小鼠炎症损伤、氧化应激和肠道菌群的影响。雄性C57BL/6小鼠随机分为正常(NC)组、模型(MC)组、菊粉(IN)组、复合菊粉(CIN)组和吡非尼酮(PFD)组。采用气管注射博来霉素的方式建立肺纤维化小鼠模型,NC组和MC组灌胃生理盐水,IN组灌胃5.00 g/kg菊粉,CIN组灌胃5.00 g/kg复合菊粉,PFD组灌胃300.00 mg/kg吡非尼酮。28 d后,为评估菊粉、复合菊粉对肺纤维化小鼠生理生化和肠道菌群结构的影响,对肺组织病理学、炎症因子、氧化应激和肠道菌群进行分析。结果表明,与MC组相比,菊粉、复合菊粉可以降低肺组织炎性细胞浸润和纤维增生;显著降低炎症因子肿瘤坏死因子-α、白介素-6和白介素-1 β水平(p<0.05);增强总抗氧化能力和提高超氧化物歧化酶活性,降低丙二醛水平;改善肠道菌群紊乱,促进杜氏杆菌属(Dubosiella)、norank_f__Muribaculaceae和阿克曼氏菌属(Akkermansia)增殖,抑制瓦伦斯回肠杆菌属(Lleibaterium)增殖。综上,菊粉或复合菊粉膳食纤维可以改善肺纤维化小鼠炎症损伤和氧化应激,调节肠道菌群紊乱。To investigate the effect of inulin or compound inulin dietary fiber on inflammatory injury,oxidative stress,and intestinal flora of mice with pulmonary fibrosis,male C57BL/6 mice were randomly divided into normal(NC)group,model(MC)group,inulin(IN)group,compound inulin(CIN)group,and pirfenidone(PFD)group.Mice models of pulmonary fibrosis were established by endotracheal injection of bleomycin,and then the NC and MC were given normal saline.IN was given 5.00 g/kg inulin,and CIN was given 5.00 g/kg compound inulin.PFD was given 300.00 mg/kg pirfenidone.After 28 d,to evaluate the effects of inulin and compound inulin on physiological and biochemical properties and intestinal flora structure of mice with pulmonary fibrosis,the pulmonary histopathology,inflammatory factor,oxidative stress,and intestinal flora were analyzed.The results showed that compared to MC,inulin and compound inulin could decrease inflammatory infiltration and fibrous hyperplasia of lung tissue,significantly decreased the level of tumor necrosis factor-α,interleukin-6,and interleukin-1β(p<0.05),bolstered the total antioxidant capacity,and enhanced the activity of superoxide dismutase.They could decrease the level of malondialdehyde,alleviate intestinal flora disturbance,promote the proliferation of Dubosiella,norank_f__Muribaculaceae,and Akkermansia,and inhibit the proliferation of Lleibaterium.In conclusion,inulin or compound inulin dietary fiber could improve inflammatory damage and oxidative stress of mice with pulmonary fibrosis and regulate intestinal flora disturbance.

关 键 词：菊粉 复合菊粉 肺纤维化 肠道菌群 炎症 氧化应激 

分 类 号：TS201.4[轻工技术与工程—食品科学]