生防菌株森吉木霉M75挥发性气体的抑菌活性分析  

Analysis of Antifungal Activity of Volatile Gas from Biocontrol Strain Trichoderma songyi M75

作　　者：张铭 朱帆 张辉[1] 程元 谢宪 张星耀[2,5] 梁军 ZHANG Ming;ZHU Fan;ZHANG Hui;CHENG Yuan;XIE Xian;ZHANG Xing-yao;LIANG Jun(Beijing Botanical Garden/Beijing Floriculture Engineering Technology Research Center,Beijing 100093,China;Key Laboratory of Forest Protection of National Forestry and Grassland Administration,Eecology and Nature Conversation Institute,Chinese Academy of Forestry,Beijing 100091,China;College of Geographical Sciences and Environment,Shijiazhuang University,Shijiazhuang 050035,Hebei,China;Agriculture Science and Engineering School,Liaocheng University,Liaocheng 252000,Shandong,China;Kunyushan Forest Ecosystem Research Station,Yantai 264100,Shandong,China)

机构地区：[1]北京市植物园管理处/北京市花卉园艺工程技术研究中心,北京100093 [2]中国林业科学研究院森林生态环境与自然保护研究所,国家林业和草原局森林保护学重点实验室,北京100091 [3]石家庄学院地理科学与环境学院,河北石家庄050035 [4]聊城大学农学与农业工程学院,山东聊城252000 [5]昆嵛山森林生态系统国家定位观测研究站,山东烟台264100

出　　处：《林业科学研究》2024年第4期127-135,共9页Forest Research

基　　金：北京市公园管理中心科技课题(ZX2024012);国家重点研发计划课题(2018YFC1200402);国家自然科学基金面上项目(31270682)。

摘　　要：[目的]解析生防菌株森吉木霉M75产生的挥发性有机化合物的成分。[方法]利用顶空固相微萃取气相色谱-质谱联用技术(HS-SPME-GC-MS)对森吉木霉(Trichoderma songyi)M75产生的挥发性气体中的有机化合物进行成分解析,并对其中的成分进行抑菌活性的检测。[结果]从森吉木霉M75菌株产生的挥发性气体中鉴定出18种化合物,包括醛类、萜类、酮类、烷烃类、芳烃类、酯类等。其中6-戊基-2H-吡喃-酮(6PP)含量最高,相对含量为18.56%,对松球壳孢菌(Sphaeropsis sapinea)抑制效果最好的化合物是芳樟醇,相对含量为5.86%,并且芳樟醇的抑菌谱较广,对多种植物病原菌如金黄壳囊菌(Cytospora chryso-sperma)、栗疫菌(Cryphonectria parastica)和链格孢(Alternria sp)也具有较好的抑制效果,抑菌率均达到70.00%以上。[结论]木霉属真菌普遍具有产挥发性有机化合物的能力,本研究成功解析了森吉木霉M75菌株产生的挥发性有机化合物,为木霉的抑菌机制及抑菌物质的代谢等研究提供科学依据,为利用木霉属真菌开发生物防治剂奠定了基础。[Objecive]To analyze the composition of volatile organic compounds produced by the biocontrol strain Trichoderma songyi M75.[Method]Using headspace solid-phase microextraction-gas chromatography-mass spectrometry(HS-SPMEGC-MS),volatile organic components of T.songyi M75 were analyzed and the antibacterial activity of some of the substance components was tested.[Result]There were 18 volatile components successfully identified from M75 strain,including aldehydes,terpenes,ketones,alkanes,aromatics and esters.The content of 6-amyl-2H-pyran-one was the highest,with a relative content of 18.56%,but linalool had the best inhibitory effect against S.sapinea,with a relative content of 5.86%.Linalool had a wide antibacterial spectrum and also had good inhibitory effects on a variety of plant pathogens such as Cytospora chrysosperma,Cryphonectria parastica and Alternria sp,with an inhibition rate of more than 70.00%.[Conclusion]Trichoderma spp generally have the ability to produce volatile organic compounds.This study successfully analyzed the volatile organic compounds produced by Trichoderma songyi M75 strain,providing scientific basis for the antibacterial mechanism of Trichoderma spp and the metabolism of antibacterial substances,and laying the foundation for the development of biocontrol agents using Trichoderma spp.

关 键 词：森吉木霉 挥发性气体 顶空固相微萃取-气质联用 成分鉴定 抑菌活性 

分 类 号：S763.15[农业科学—森林保护学]