万州零售淡水鱼中副溶血性弧菌的污染状况与特征研究  

Study on pollution status and characteristics of Vibrio parahaemolyticus inretail freshwater fish in Wanzhou

作　　者：赵力 丁国英 朱秋昊 刘奇 黄玲冬 肖国生 赵峰 ZHAO Li;DING Guoying;ZHU Qiuhao;LIU Qi;HUANG Lingdong;XIAO Guosheng;ZHAO Feng(College of Biology and Food Engineering,Chongqing Three Gorges University,Chongqing 404100,China)

机构地区：[1]重庆三峡学院生物与食品工程学院,重庆404100

出　　处：《食品与发酵工业》2024年第16期242-248,共7页Food and Fermentation Industries

基　　金：重庆市教委科研项目(KJQN202001231);万州区2022年科技计划项目。

摘　　要：为了解万州零售淡水鱼中副溶血性弧菌(Vibrio parahaemolyticus,Vp)污染状况、血清型、耐药性及亲缘关系,于2022年—2023年采集万州零售淡水鱼共141份,根据GB 4789.7—2013《食品安全国家标准食品微生物学检验副溶血性弧菌检验》分离Vp和定量,PCR法特异性扩增tlh、tdh、trh、toxR、pirA、T3SS1、T3SS2和T3SS2基因,用多重PCR检测血清型,纸片扩散法检测耐药性以及肠杆菌科基因间重复序列(enterobacterial repetitive intergenic consensus, ERIC)-PCR分析亲缘关系。结果显示,141份样品中总污染率为38.3%,阳性样品定量范围为1.5~1 100 MPN/g,花鲢污染率最高(66.7%)。Vp受季节和温度的影响,夏季污染率最高(57.1%)。54株Vp全部携带tlh、toxR和T3SS1基因,1株同时携带tdh和T3SS2α基因,未检测到trh、pirA和T3SS2β基因。血清分布较分散,分为9个O群,O1为优势群(33.3%)。54株Vp对苯唑西林、青霉素和氨苄西林耐药率分别为:100%、98.1%和90.7%,对头孢他啶、头孢曲松、庆大霉素、四环素、多西环素和米诺环素100%敏感。ERIC-PCR分析表明,存在与临床分离株有遗传相似的致病菌株。该研究结果可为淡水鱼养殖、消费和临床治疗Vp导致的食源性疾病提供理论依据。To investigate the prevalence,serotype,antibiotic resistance,and genetic relationship of Vibrio parahaemolyticus(Vp)in the retail freshwater fish in Wanzhou,141 samples of retail freshwater fish were collected from 2022 to 2023.The Vp was isolated and quantified according to the method of GB 4789.7—2013,and then PCR was used to amplify the thermolabile hemolysin gene(tlh),heat-resistant direct hemolysin gene(tdh),heat-resistant-related hemolysin gene(trh),transmembrane transcriptional regulator gene(toxR),Photorhabdus insect-related toxin gene(pirA),typeШsecretion system 1 gene(T3SS1),and typeШsecretion system 2 genes(T3SS2αand T3SS2β)that were associated with acute hepatopancreas necrosis.Multiplex PCR was used to detect serotypes.The Kirby-Bauer and enterobacterial repeat intergenic consensus sequence PCR(ERIC-PCR)were used to detect antimicrobial susceptibility and genetic relationship,respectively.Results showed that 54 samples were Vp positive out of 141 samples(38.3%),When the quantitative range of positive samples was in the range of 1.5 MPN/g to 1100 MPN/g,the pollution of Aristichys nobilis was the highest(66.7%).Temperature significantly affected the abundance of Vp,and the seasonal differences in sample pollution were significant,with the highest pollution rate of 57.1%in summer.All 54 Vp isolates carried tlh,toxR,and T3SS1 genes,one isolate carried both tdh and T3SS2αgenes,and trh,pirA,and T3SS2βgenes were not found.The serotypes distribution was more dispersed and divided into nine O groups,with an O1 dominant cluster(33.3%).The resistance rates of 54 Vp strains to benzoxacillin,penicillin,and ampicillin were 100%,98.1%,and 90.7%,respectively.54 Vp strains were 100%susceptible to ceftazidime,ceftriaxone,gentamicin,tetracycline,doxycycline,and minocycline.ERIC-PCR showed that the isolate carrying pathogenic genes was genetically similar to clinical isolates.The results of this study might provide a theoretical basis for freshwater fish farming,consumption and clinical treatment of foodborne di

关 键 词：副溶血性弧菌 淡水鱼 毒力基因 血清型 耐药性 ERIC-PCR 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]