蜂蜜中链霉素仿生印迹电化学传感检测方法  

Biomimetic imprinted electrochemical sensing detection of streptomycin in honey

作　　者：李兆周[1,2,3] 赵明辉 张冉 陈秀金 王耀[1,2,3] 万宁波 牛华伟[1,2,3] 高红丽 李芳 于慧春 袁云霞[1,2,3] 吴影 李佩艳[1,2,3] LI Zhaozhou;ZHAO Minghui;ZHANG Ran;CHEN Xiujin;WANG Yao;WAN Ningbo;NIU Huawei;GAO Hongli;LI Fang;YU Huichun;YUAN Yunxia;WU Ying;LI Peiyan(College of Food and Bioengineering,Henan University of Science and Technology,Luoyang 471000,China;Henan International Joint Laboratory of Food Green Processing and Quality Safety Control,Luoyang 471000,China;National Demonstration Center for Experimental Food Processing and Safety Education,Luoyang 471000,China)

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471000 [2]河南省食品绿色加工与质量安全控制国际联合实验室,河南洛阳471000 [3]食品加工与安全国家级实验教学示范中心,河南洛阳471000

出　　处：《食品与发酵工业》2024年第16期285-292,共8页Food and Fermentation Industries

基　　金：洛阳市公益性行业科研专项(2202021A);河南省科技攻关项目(242102411001,232102320298);河南省优秀青年科学基金(202300410121);国家自然科学基金(31701694,U1504330,31702218);河南省青年人才托举工程项目(2020HYTP029);河南省研究生教育改革与质量提升工程项目(HNYJS2020JD06)。

摘　　要：动物源食品中链霉素残留危害严重,建立高性能分析方法是确保食品安全和人类健康的必要手段。仿生印迹电化学传感检测方法具有高效、特异和灵敏等优点,已成为食品安全检测技术研究的重要领域。该研究以链霉素为模板分子,氧化石墨烯和壳聚糖为增敏材料,3-氨基苯基硼酸和邻苯二胺为复合功能单体,采用电聚合法构建了仿生印迹聚合物传感界面,建立了蜂蜜中链霉素的仿生印迹电化学传感检测方法。通过扫描电镜对印迹传感界面进行了表征,采用循环伏安法、差分脉冲伏安法和电化学交流阻抗法对仿生印迹传感器的电化学性能进行表征。在最佳反应条件下,该传感器的检测限为2.59×10^(-12) mol/L,线性范围为1.0×10^(-10)~1.0×10^(-6) mol/L,洋槐蜜、枣花蜜和桂花蜜样品的加标回收率分别为94.78%~106.24%、93.65%~103.81%和97.19%~106.18%,相对标准偏差≤4.28%。试验结果表明,该方法具有良好的重复性、稳定性和重现性,能够满足蜂蜜中链霉素残留分析的需要。相关研究为复杂基质中痕量靶标的高特异性和超灵敏分析提供了技术支撑,对于蜂蜜中链霉素的快速筛查和安全监测具有重要意义。There has been a serious hazard for streptomycin residues in animal-derived foods.It is necessary to establish a high-performance analytical method to ensure food safety and human health.The biomimetic imprinting electrochemical sensing detection method has the advantages of high efficiency,specificity,and sensitivity,and has become an important area of research in the field of food safety detection technology.In this study,a biomimetic imprinted sensing interface was constructed by electropolymerization with streptomycin as the template,graphite oxide and chitosan as the sensitizing materials,and 3-aminobenzeneboronic acid and O-phenylenediamine as the composite functional monomers.The biomimetic imprinted electrochemical sensing detection method was then developed and applied for STR residue analysis in honey.The imprinted interface was characterized by scanning electron microscopy,and the electrochemical performance of the proposed sensor was evaluated with cyclic voltammetry,differential pulse voltammetry,and electrochemical impedance analysis.Under the optimal reaction conditions,the linear range was calculated as 1.0×10^(-10)-1.0×10^(-6) mol/L with a detection limit of 2.59×10^(-12) mol/L,the recoveries of sophora nectar,jujube nectar,and osmanthus nectar were 94.78%-106.24%,93.65%-103.81%,and 97.19%-106.18%,respectively,and the relative standard deviation was less than or equal to 4.28%.The experimental results show that this method has good repeatability,stability,and reproducibility.The related research provides technical support for the high specificity and ultrasensitive analysis of trace target compounds in complex matrices,which is of great significance for the rapid screening and safety monitoring of streptomycin in honey.

关 键 词：蜂蜜 链霉素 仿生印迹 电化学传感器 

分 类 号：S896.1[农业科学—特种经济动物饲养] O657.1[农业科学—畜牧兽医]