再生丝素蛋白在Na+溶液中的分子聚集行为及生物学性能研究  

Molecular Aggregation Behavior and Biological Properties of Regenerated Silk Fibroin in Na+Solution

作　　者：应勇 汪芸萱 郭蕾 徐玉玲 张军涛 许承志 未本美 汪海波 YING Yong;WANG Yunxuan;GUO Lei;XU Yuling;ZHANG Juntao;XU Chengzhi;WEI Benmei;WANG Haibo(School of Chemical&Environmental Engineering,Wuhan Polytechnic University,Wuhan 430023,China;College of Life Science and Technology,Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables,Hubei Engineering University,Xiaogan 432000,China)

机构地区：[1]武汉轻工大学化学与环境工程学院,湖北武汉430023 [2]湖北工程学院生命科学与技术学院特色果蔬质量控制湖北省重点实验室,湖北孝感432000

出　　处：《食品工业科技》2024年第17期65-72,共8页Science and Technology of Food Industry

基　　金：武汉市知识创新专项项目-基础研究项目(2023020201010148);国家自然科学基金项目(22378320,22178277)。

摘　　要：目的:丝素蛋白在特定条件下的聚集行为能够影响其动力学行为及黏弹性能,进而影响丝素蛋白材料的生物学性能,因此,本文通过系统研究丝素蛋白的自组装聚集行为,进而研究增强其力学性能及生物学性能的条件。方法:以家蚕蚕茧为原料,提取制备得到再生丝素蛋白,通过浊度实验寻找最佳组装条件;丝素蛋白溶液加入Na+后,通过流变学实验研究其对动力学行为和黏弹性能的影响;通过细胞增殖实验研究丝素蛋白的生物学性能。结果:提取的再生丝素蛋白以无规卷曲结构为主;最佳体外自组装条件:浓度为4 mg/mL的溶液在70℃组装6 h;丝素蛋白溶液中加入Ca^(2+)对体外自组装起抑制作用,而加入Na^(+)可以加快自组装速率和提高组装程度,并且随着Na+浓度的增加,丝素蛋白凝胶强度逐渐增强,凝胶网络更稳定;细胞增殖实验显示,再生丝素蛋白溶液比其自组装纤维更利于细胞生长和增殖。结论:丝素蛋白的浓度、温度、组装时间和离子都会对丝素蛋白的体外自组装聚集行为产生影响,本文初步研究丝素蛋白的自组装聚集条件和生物学性能,为提高丝素蛋白作为食品包装材料的性能提供实验依据。Objective:The aggregation behavior of silk fibroin under specific conditions can influence its kinetic behavior and viscoelastic properties,thereby affecting biological properties of silk fibroin materials.Therefore,this study systematically investigated the self-assembly aggregation behavior of silk fibroin and explored the conditions for enhancing its mechanical performance and biological properties.Methods:Regenerated silk fibroin was extracted and prepared from domestic silkworm cocoons,and the optimal assembly conditions were determined through turbidity experiments.Rheological experiments were conducted to study the influence of Na+on the kinetic behavior and viscoelastic properties of silk fibroin solutions.Cell proliferation experiments were carried out to investigate the biological properties of silk fibroin.Results:The extracted regenerated silk fibroin primarily exhibited a random coiled structure.The optimal in vitro self-assembly conditions involved assembly of a 4 mg/mL solution at 70℃for 6 hours.The addition of Ca^(2+)to the silk fibroin solution inhibited in vitro self-assembly,while the addition of Na+accelerated the assembly rate and increased the degree of assembly.Furthermore,with increasing Na+concentration,the gel strength of silk fibroin gradually increased,leading to a more stable gel network.Cell proliferation experiments demonstrated that the solution of regenerated silk fibroin was more conducive to cell growth and proliferation than its self-assembled fibers.Conclusion:The concentration,temperature,assembly time,and ions all have an impact on the in vitro self-assembly aggregation behavior of silk fibroin.This study provides preliminary research on the self-assembly aggregation conditions and biological properties of silk fibroin,offering experimental basis for improving the performance of silk fibroin as a food packaging material.

关 键 词：再生丝素蛋白 自组装 流变学 黏弹性能 组装程度 

分 类 号：TS201.2[轻工技术与工程—食品科学]