响应面法优化微酸性电生功能水萌发苦荞芽工艺及其富硒规律研究  

Optimization of Germination of Tartary Buckwheat with Slightly Acidic Electrolyzed Water by Response Surface Methodology and Its Selenium-enriched Rules

作　　者：赵一迪 刘媛 张凯 尹晨 朱伟冉 王健 李云龙 周海涛 牟建楼 ZHAO Yidi;LIU Yuan;ZHANG Kai;YIN Chen;ZHU Weiran;WANG Jian;LI Yunlong;ZHOU Haitao;MOU Jianlou(Hebei Key Laboratory of Quality and Safety Analysis of Agricultural Products and Food,Hebei North University,Zhangjiakou 075000,China;Shanxi Functional Food Research Institute,Shanxi Agricultural University,Taiyuan 030031,China;College of Food Science and Technology,Agricultural University of Hebei,Baoding 071000,China;Zhangjiakou Academy of Agricultural Sciences,Zhangjiakou 075000,China)

机构地区：[1]河北北方学院河北省农产品食品质量安全分析检测重点实验室,河北张家口075000 [2]山西农业大学山西功能食品研究院,山西太原030031 [3]河北农业大学食品科技学院,河北保定071000 [4]张家口市农业科学院,河北张家口075000

出　　处：《食品工业科技》2024年第17期155-162,共8页Science and Technology of Food Industry

基　　金：山西省重点研发计划项目(201903D211006);张家口市重点研发计划项目(1911016-C);国家现代农业(燕麦荞麦)产业技术体系建设专项(CARS07-E2-04)。

摘　　要：为探究不同pH、有效氯浓度(Available Chlorine Contents,ACC)的微酸性电生功能水(Slightly Acidic Electrolyzed Water,SAEW)萌发条件对发芽苦荞的影响规律,以发芽率为考察指标,通过单因素和响应面试验优化微酸性电生功能水萌发苦荞芽工艺;为获得总硒含量高且品质优良的富硒苦荞芽,以总硒含量和发芽率为考察指标,在微酸性电生功能水萌发基础上,研究了不同亚硒酸钠浓度对苦荞发芽的影响。结果表明,最佳工艺参数为:浸泡时间为2.3 h,pH为6.5,萌发温度为21℃,萌发时间为108 h,在该条件下发芽率为84.96%,与响应面预测值(85.36%)接近,相比蒸馏水组提高了14.3%,比自来水组提高了11.71%。优选的Na2SeO3溶液浓度为60 mg/L,苦荞芽的总硒含量为15.5 mg/L且发芽率为85.63%,高于响应面优化预测值且高于苦荞发芽实际值。本研究为微酸性电生功能水和富硒处理萌发苦荞芽产品的开发提供了理论基础和科学依据。To explore the influence of slightly acidic electrolyzed water(SAEW)with different pH and available chlorine contents(ACC)on the germination of tartary buckwheat(Fagopyrum tataricum),the germination rate was taken as the index,and the germination process of tartary buckwheat sprouts with SAEW was optimized by single factor and response surface experiments.Moreover,to obtain good quality selenium-enriched tartary buckwheat sprouts,the effects of different Na2SeO3 concentrations on tartary buckwheat were studied on the basis of SAEW germination,with the total selenium content and germination rate as indexes.The optimum technological parameters were determined as follows:soaking time 2.3 h,pH6.5,germination temperature 21℃and germination time 108 h.Under these conditions,the germination rate was 84.96%,which was close to the predicted value of the response surface group(85.36%),being 14.3%and 11.71%higher than those of distilled water and tap water groups,respectively.The optimal concentration of Na2SeO3 was 60 mg/L,under which the total selenium content of tartary buckwheat sprouts was 15.5 mg/L,and the germination rate was 85.63%,which were both higher than the predicted and actual values of response surface group.This study provided a theoretical and scientific basis for the development of SAEW and selenium-enriched treatment of tartary buckwheat sprouts.

关 键 词：微酸性电生功能水 苦荞 发芽 响应面法 工艺优化 富硒 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]