Amphipathicity mediated endocytosis of mesoporous silica nanoparticles with tunable frameworks  

作　　者：Runfeng Lin Tiancong Zhao Liang Chen Minchao Liu Hongyue Yu Ruicong Wang Minjia Yuan Xiaomin Li Dongyuan Zhao 

机构地区：[1]Department of Chemistry,State Key Laboratory of Molecular Engineering of Polymers,iChem,Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials,Fudan University,Shanghai 200433,China [2]Shanghai Qiran Biotechnology Co.,Ltd.,Shanghai 201702,China

出　　处：《Nano Research》2024年第9期8350-8359,共10页纳米研究（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.22075049,22305042,22305041,21875043,22088101,21905052,and 51961145403);the National Key R&D Program of China(No.2018YFA0209401);the Key Basic Research Program of Science and Technology Commission of Shanghai Municipality(No.22JC1410200);the Natural Science Foundation of Shanghai(Nos.22ZR1478900 and 20490710600);the Shanghai Science and Technology Innovation Action Plan Morning Star Project(Sail Special,Nos.20QA1401200,22YF1402200,and 23YF1401900);the Shanghai Pilot Program for Basic Research-Fudan University(No.22TQ004);the Fundamental Research Funds for the Central Universities(No.20720220010).

摘　　要：Due to the amphiphilic nature of phospholipids in the cell membrane,the amphipathicity of the nanomedicine plays a crucial role in the endocytosis.However,limited biological characterization methods restrict the study of the state of nanoparticles with different amphiphilicities on cell membranes.The understanding of interaction of amphiphilic particle with cell membrane is still lacking.Herein,by combining the dissipative particle dynamics(DPD)with the framework construction of mesoporous silica nanoparticles(MSNs),we demonstrate the enhanced endocytosis induced by the hydrophobicity.DPD results confirm that the presence of hydrophobic groups on the surface of nanoparticles can disturb the integrity of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine(POPC)membrane and induce activation of phospholipids to a higher energy level,thereby facilitating the wrapping of nanoparticles.To validate the simulation findings,uniform MSNs with hydrophilic pure silica framework and two types of amphiphilic MSNs with varying hydrophilic organic groups in the framework are rationally synthesized by using different silane precursors.The obtained three kinds of MSNs show similar diameter(~100 nm)and mesopores(~2 nm),but distinct hydrophobicity/hydrophilicity ratio.The phenyl-bridged MSN with a carbon content of 27.1%exhibits enhanced cellular uptake,consistent with the theoretical simulation results.This work sheds light on how the surface amphipathicity influences endocytosis through the interaction with cell membrane.

关 键 词：mesoporous silica nanoparticles mesoporous organosilica ENDOCYTOSIS cell membrane AMPHIPATHICITY 

分 类 号：TB383[一般工业技术—材料科学与工程]