基于测序及近缘种间比较分析滴水珠叶绿体基因组特征  

Plastome Characteristics of Pinellia cordata(Araceae)Revealed by Sequencing and Comparative Analyses with Related Species

作　　者：罗须 罗睿[1,2] 杜声珊 姚妹 王雨 Luo Xu;Luo Rui;Du Shengshan;Yao Mei;Wang Yu(College of Life Science,Guizhou University,Guiyang,550025;Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Guizhou University,Guiyang,550025)

机构地区：[1]贵州大学生命科学学院,贵阳550025 [2]贵州大学,山地植物资源保护与保护种质创新教育部重点实验室,贵阳550025

出　　处：《分子植物育种》2024年第16期5297-5306,共10页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31960067;31560081);贵州省自然科学基金项目(Z073181)共同资助。

摘　　要：为确认滴水珠叶绿体基因组的大小、特征及与近缘种的关系,本研究对滴水珠叶绿体基因组进行测序和拼接。发现其总长为164850 bp,GC含量为36.5%;具有典型的环状四分区结构,包括一个大单拷贝区(LSC,89964 bp)、一个小单拷贝区(SSC,25120 bp)和一对反向重复序列(IRs,24883 bp);共注释到113个特异基因,包括79个蛋白编码基因、30个tRNA基因和4个rRNA基因;共检测到331个SSR位点,且大多位点均具有A/T碱基偏好性。经叶绿体基因组序列比对发现,7个天南星科物种在叶绿体基因组结构、基因顺序和基因含量等均具有高度保守性。IR区边界扩张与收缩分析发现,滴水珠叶绿体基因组只含有一个单拷贝的trnN-GUU基因,其IR区较其他物种发生了收缩。系统发育分析发现,半夏属形成一个单系,且滴水珠的分枝长度比其他两个物种长。本研究提供了天南星科植物的叶绿体基因组序列信息,且发现相对于半夏属的其他物种滴水珠可能具有特殊的进化历史。To clarify the size,characteistics of the chloroplast genome in P.cordata and the relationship of related species.Here,we had sequenced and assembled the complete plastome of P.cordata.This plastome(164850 bp)is a typical double-stranded circular quadripartite structure,with 36.5%GC.A pair of inverted repeat(IRs,24883 bp)were separated by a large single-copy(LSC,89964 bp)region and a small single-copy(SSC,25120 bp)region.The whole plastome encoded 113 unique genes,including 79 protein-coding,30 tRNA and 4 rRNA genes.In addition,a total of 331 simple sequence repeats(SSR),most of which were A/T motifs,were identified in this plastome.Based on comparative analysis with seven plastomes of Araceae,highly conservation in genome structure,gene order,and gene content were showed.The results of the contraction and expansion of IR regions at the junction analysis that there was only one copy of trnN-GUU gene and a specific IR contraction.Consequently,the branch length of P.cordata was longer than other species in the Pinellia monophyletic groups revealed by phylogenetic trees.Taken together,our results provided more plastome information in Araceae and indicated that P.cordata should possess special evolutionary history in comparison to other speices in Pinellia.

关 键 词：叶绿体基因组 天南星科 半夏属 滴水珠 系统进化树 

分 类 号：S567.239[农业科学—中草药栽培]