口腔鳞癌(OSCC)患者口腔微生群落特征及临床相关意义  

Characterization and clinical relevance of oral microbiota in patients with oral squamous carcinoma

作　　者：葛益林 宋喜 宋辉 成嘉明 李殷 GE Yilin;SONG Xi;SONG Hui;CHENG Jiaming;LI Yin(Department of Otolaryngology,Head and Neck Surgery,Affiliated Changsha Hospital of Xiangya Medical College,Central South University(Changsha First Hospital),Changsha 450001,China)

机构地区：[1]中南大学湘雅医学院附属长沙医院(长沙市第一医院)耳鼻咽喉头颈外科,湖南长沙450001

出　　处：《中国病原生物学杂志》2024年第9期1020-1024,1030,共6页Journal of Pathogen Biology

基　　金：湖南省卫生健康委科研计划项目(No.202107011445)。

摘　　要：目的探究口腔鳞状细胞癌(OSCC)患者与健康对照组之间口腔微生物组的差异,并分析这些微生物群落的变化与OSCC的临床特征之间的关系。方法本研究纳入80名经病理确认的OSCC患者,从每位患者的癌变部位及相应健康口腔位置采集样本。采用高通量16S rRNA基因测序分析微生物组成,并运用QIIME2软件进行微生物群落的分类学分析。通过α多样性与β多样性评估,以及LEfSe分析用于识别具有统计学意义的微生物差异。结果OSCC患者的口腔微生物α多样性显著低于对照组,具体表现为Chao1指数(31.22±7.14 vs.50.25±8.39,P<0.05)、Shannon指数(1.95±0.39 vs.3.08±0.49,P<0.05)和Simpson指数(0.70±0.06 vs.0.90±0.06,P<0.05)。β多样性分析显示两组间微生物组成存在明显分离。LEfSe分析识别出OSCC组中显著增多的微生物属包括Prevotella、Porphyromonas和Fusobacterium。功能预测分析指出OSCC相关微生物群落在病毒感染机制和癌症相关通路中富集。结论OSCC患者的口腔微生物多样性显著降低,这些微生物的变化可能与OSCC的发展密切相关,未来研究需探讨这些微生物变化与OSCC临床特征之间的具体联系,以及其潜在的治疗应用。Objective To examine the differences in the oral microbiome between patients with oral squamous cell carcinoma(OSCC)and healthy controls,and to analyze the relationship between changes in these microbial communities and the clinical characteristics of OSCC.Methods This study included 80 pathologically confirmed OSCC patients,and samples were collected from each patient's cancerous site and corresponding healthy oral location.High-throughput 16S rRNA gene sequencing was used to analyze microbial composition,and QIIME2 software was used to conduct taxonomic analysis of microbial communities.Alpha and beta diversity assessments,and LEfSe analysis were used to identify statistically significant microbial differences.Results The alpha diversity of oral microorganisms in OSCC patients was significantly lower than that in the control group,as shown by Chao1 index(31.22±7.14 vs.50.25±8.39,P<0.05),Shannon index(1.95±0.39 vs.3.08±0.49,P<0.05)and Simpson index(0.70±0.06 vs.0.90±0.06,P<0.05).Beta diversity analysis showed a clear separation in microbial composition between the two groups.LEfSe analysis identified significantly increased microbial genera in the OSCC group including Prevotella,Porphyromonas,and Fusobacterium.Functional prediction analysis pointed out that OSCC-related microbial communities were enriched in viral infection mechanisms and cancer-related pathways.Conclusion The oral microbial diversity of patients with OSCC is significantly reduced.These microbial changes may be closely related to the development of OSCC.Future research needs to explore the specific connection between these microbial changes and the clinical characteristics of OSCC,as well as its potential therapeutic application.

关 键 词：口腔鳞状细胞癌(OSCC) 微生物多样性 16S rRNA基因测序 微生物组 

分 类 号：R739.8[医药卫生—肿瘤]