The transgenerational effects of maternal low-protein diet during lactation on offspring  

作　　者：Lin-Jian Gu Li Li Qian-Nan Li Ke Xu Wei Yue Jing-Yi Qiao Tie-Gang Meng Ming-Zhe Dong Wen-Long Lei Jia-Ni Guo Zhen-Bo Wang Qing-Yuan Sun 

机构地区：[1]State Key Laboratory of Stem Cell and Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Institute of Laboratory Animal Sciences,CAMS&PUMC,Beijing 100021,China [4]Guangzhou Key Laboratory of Metabolic Diseases and Reproductive Health,Guangdong-Hong Kong Metabolism&Reproduction Joint Laboratory,Reproductive Medicine Center,Guangdong Second Provincial General Hospital,Guangzhou,Guangdong 510317,China

出　　处：《Journal of Genetics and Genomics》2024年第8期824-835,共12页遗传学报（英文版）

基　　金：supported by the National R&D program of China(2022YFC2703500);the Science and Technology Program of Guangzhou,China(202201020292 and 2023A03J0258);the Natural Science Foundation of Shandong Province(ZR2021ZD33);the Strategic Collaborative Research Program of the Ferring Institute of Reproductive Medicine,Ferring Pharmaceuticals and Chinese Academy of Sciences(FIRMC181101).

摘　　要：Environmental factors such as diet and lifestyle can influence the health of both mothers and offspring.However,its transgenerational transmission and underlying mechanisms remain largely unknown.Here,using a maternal lactation-period low-protein diet(LPD)mouse model,we show that maternal LPD during lactation causes decreased survival and stunted growth,significantly reduces ovulation and litter size,and alters the gut microbiome in the female LPD-F1 offspring.The transcriptome of LPD-F1 metaphase II(MII)oocytes shows that differentially expressed genes are enriched in female pregnancy and multiple metabolic processes.Moreover,maternal LPD causes early stunted growth and impairs metabolic health,which is transmitted over two generations.The methylome alteration of LPD-F1 oocytes can be partly transmitted to the F2 oocytes.Together,our results reveal that LPD during lactation transgenerationally affects offspring health,probably via oocyte epigenetic changes.

关 键 词：Lactationdiet Maternal low-proteindiet DNAMETHYLATION Transgenerational effect OOCYTE 

分 类 号：Q343[生物学—遗传学]