复合膳食纤维对糖尿病小鼠血糖血脂的调节及肠道菌群的影响  

Effect of complex dietary fiber on hyperglycemia,blood lipid and gut microbiota of diabetic mice

作　　者：吕权洪 刘良忠 朱哲 蔡全韬 LYU Quanhong;LIU Liangzhong;ZHU Zhe;CAI Quantao(School of Food Science and Engineering,Wuhan Polytechnic University,Wuhan 430023,China;Wuhan Longfengyuan Biotechnology Co.,Ltd.,Wuhan 430040,China)

机构地区：[1]武汉轻工大学,食品科学与工程学院,武汉430023 [2]武汉隆丰园生物科技有限公司,武汉430040

出　　处：《武汉轻工大学学报》2024年第4期12-22,共11页Journal of Wuhan Polytechnic University

基　　金：国家重点研发计划项目(编号:SQ2018YFC200044)。

摘　　要：本研究旨在探讨复合膳食纤维对糖尿病小鼠血糖血脂的调节作用,以及其对短链脂肪酸及肠道菌群的影响。选取SPF级C57BL/6J小鼠,空白组喂养基础饲料,其余小鼠连续8周喂养高糖高脂饲料后,腹腔注射链脲佐菌素(streptozotocin,STZ)溶液,建立糖尿病小鼠模型。造模成功后的小鼠随机分为模型对照组、复合膳食纤维低、中、高剂量组。空白组继续喂养基础饲料,模型对照组喂养高糖高脂饲料,复合膳食纤维低、中、高剂量组分别用含2.5%、5.0%、7.5%复合膳食纤维的高糖高脂饲料喂养8周后,模型对照组小鼠改用基础饲料喂养,复合膳食纤维低、中、高剂量组改用含2.5%、5.0%、7.5%复合膳食纤维的基础饲料喂养,连续4周,饲养结束后检测血糖血脂相关指标、短链脂肪酸含量及肠道菌群组成。结果表明:复合膳食纤维可以降低糖尿病小鼠空腹血糖,减轻胰岛素抵抗,改善血脂异常;此外,复合膳食纤维可在一定程度上促进乙酸、丙酸、丁酸、戊酸、异丁酸、异戊酸的分泌;下调Firmicutes、Rikenellaceae、Lachnospiraceae相对丰度,上调Bacteroidotas相对丰度,降低F/B值。复合膳食纤维可以有效改善糖尿病小鼠血糖血脂水平,其机制可能是通过调节肠道菌群组成以促进短链脂肪酸(SCFAs)分泌实现的。This study aimed to investigate the regulatory effects of complex dietary fiber on blood glucose,blood lipids,short-chain fatty acids(SCFAs),and gut microbiota in diabetic mice.SPF-grade C57BL/6J mice were selected,with the control group fed a basal diet while the remaining mice were fed a high-sugar,high-fat diet for 8 weeks followed by intraperitoneal injection of streptozotocin(STZ)solution to induce diabetes.The diabetic mice were randomly divided into model control group,low,medium,and high-dose complex dietary fiber groups.The control group continued on the basal diet,the model control group continued on the high-sugar,high-fat diet,while the low,medium,and high-dose complex dietary fiber groups were fed a high-sugar,high-fat diet containing 2.5%,5.0%,and 7.5%composite dietary fiber,respectively,for 8 weeks.Afterward,the model control group switched to the basal diet,and the low,medium,and high-dose complex dietary fiber groups switched to a basal diet containing 2.5%,5.0%,and 7.5%complex dietary fiber,respectively,for 4 weeks.Blood glucose,blood lipids,SCFAs content,and gut microbiota composition were assessed at the end of the feeding period.The results showed that complex dietary fiber could decrease fasting blood glucose levels,alleviate insulin resistance,and improve blood lipid abnormalities in diabetic mice.Additionally,complex dietary fiber could promote the secretion of acetic acid,propionic acid,butyric acid,pentanic acid,isobutyric acid,and isovaleric acid to some extent.It downregulated the relative abundance of Firmicutes,Rikenellaceae and Lachnospiraceae,pregulated Bacteroidotas relative abundance,reduced the F/B ratio.Complex dietary fiber effectively improved blood glucose and blood lipid levels in diabetic mice,possibly through modulation of gut microbiota composition and promotion of SCFAs secretion.

关 键 词：复合膳食纤维 糖尿病 血糖 血脂 短链脂肪酸 肠道菌群 

分 类 号：TS201.4[轻工技术与工程—食品科学]