海洋食品酶解物改善精神疲劳功能性评价的综合实验设计  

Comprehensive experimental design for the functional evaluation of marine food enzymatic hydrolysates in alleviating mental fatigue

作　　者：张恬恬 马磊[1] 邹宏宇 王成成 薛长湖[1] 王玉明[1] ZHANG Tiantian;MA Lei;ZOU Hongyu;WANG Chengcheng;XUE Changhu;WANG Yuming(College of Food Science and Engineering,Ocean University of China,Qingdao 266404,China)

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266404

出　　处：《实验技术与管理》2024年第8期36-42,共7页Experimental Technology and Management

基　　金：国家重点研发计划项目(2023YFC2812400,2023YFF1105305);山东省本科教改项目(M2023231);中国海洋大学本科教育教学研究项目(2024ZD15,2023JY066)。

摘　　要：该文设计了以小鼠模型评价海洋食品酶解物对改善精神疲劳营养功能的创新性综合实验,以海参和牡蛎两种海洋生物资源为原料,通过酶解法制备了海参酶解物和牡蛎酶解物,测定了两种酶解物的氨基酸组成,探究了两种酶解物对皮质酮所致精神疲劳模型小鼠耐力、焦虑样行为、抑郁样行为以及神经递质水平的影响。结果表明海参酶解物和牡蛎酶解物均可显著提高精神疲劳小鼠的耐力表现,并改善焦虑样行为和抑郁情绪,其中,海参酶解物在改善焦虑样行为方面更显著,而牡蛎酶解物在改善抑郁情绪方面更显著。进一步研究发现两种酶解物通过调节神经递质水平改善精神疲劳。该综合实验结合了海洋生物资源精深加工技术、食品检验与分析、食品营养学、功能性食品等多领域实验技术,有助于提高学生的学习积极性、深化学生所学理论知识、培养其创新思维和实验技能。[Objective]Long-term fatigue increases the risk of various diseases and poses a serious threat to physical and mental health.Marine foods contain valuable,high-quality proteins,and sea cucumbers and oysters are rich in nutritional ingredients.Although various studies have shown that enzymatic hydrolysates from sea cucumbers and oysters have antifatigue nutritional benefits,current research mostly focuses on their role in combating physical fatigue,with no reported effects on mental fatigue.In this study,an innovative,comprehensive experiment was designed to evaluate the functionality of sea cucumber and oyster enzymatic hydrolysates in alleviating mental fatigue using a mouse model.[Methods]Enzymatic hydrolysates of sea cucumbers and oysters were prepared through enzymatic hydrolysis using complex protein enzymes,and their amino acid compositions were measured using an automated amino acid analyzer.Moreover,a mouse model of mental fatigue was established by administering corticosterone via subcutaneous injection for 4 weeks.The mental fatigue mice were divided into three groups:the model group,the sea cucumber hydrolysate group,and the oyster hydrolysate group.Normal mice were used as the control group.The dose of hydrolysates administered orally was 200 mg/kg body weight.The effects of sea cucumber and oyster hydrolysates on endurance,anxiety-like behavior,and depression-like behavior were investigated through exhaustive running tests,open field tests,elevated plus maze tests,and tail suspension tests.Moreover,neurotransmitter levels in the brain were measured to elucidate possible related mechanisms.[Results]The results showed that the amino acids with the highest content in the sea cucumber hydrolysate were glycine,glutamic acid,aspartic acid,arginine,and proline,while the amino acids with the highest content in the oyster hydrolysate were glutamic acid,taurine,aspartic acid,and lysine.Dietary supplementation with sea cucumber and oyster hydrolysate considerably enhanced endurance performance,leading to a 27.6

关 键 词：海洋食品 精神疲劳 高值化利用 综合实验 

分 类 号：TS201.3[轻工技术与工程—食品科学]