环圈链霉菌(Streptomyces anulatus)89-2-2全基因组测序及序列分析  

Whole-genome sequencing and sequence analysis of Streptomyces anulatus 89-2-2

作　　者：阮文伟 付建红 崔凤真[1,2,3] 铁瑞岚 徐国燕 阿依卡买尔·艾克拜尔 RUAN Wenwei;FU Jianhong;CUI Fengzhen;TIE Ruilan;XU Guoyan;Ayekabayr Ekbayr(Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology,Urumqi 830017,Xinjiang,China;Key Laboratory of Plant Stress Biology in Arid Land,Urumqi 830017,Xinjiang,China;College of Life Sciences,Xinjiang Normal University,Urumqi 830017,Xinjiang,China)

机构地区：[1]新疆特殊环境物种保护与调控生物学实验室,新疆乌鲁木齐830017 [2]干旱区植物逆境生物学实验室,新疆乌鲁木齐830017 [3]新疆师范大学生命科学学院,新疆乌鲁木齐830017

出　　处：《微生物学通报》2024年第8期3085-3102,共18页Microbiology China

基　　金：国家自然科学基金(32260226);新疆维吾尔自治区“天山”创新团队计划(2022D14004);新疆维吾尔自治区自然科学基金(2021D01A122)。

摘　　要：【背景】环圈链霉菌(Streptomyces anulatus)89-2-2是一株中度嗜盐放线菌,其次级代谢产物具有抑制蘑菇酪氨酸酶活性、抑制小鼠黑色素瘤B16细胞内酪氨酸酶活性及黑色素合成的作用。目前S.anulatus 89-2-2基因组序列分析尚未见报道,这限制了该菌株中酪氨酸酶抑制剂、黑色素合成抑制剂及其他次级代谢产物合成及调控的研究。【目的】解析S.anulatus 89-2-2基因组序列信息,挖掘其次级代谢产物基因资源,为深入研究该菌株的酪氨酸酶抑制剂产生机理及生物合成调控机制奠定基础。【方法】利用Nanopore测序平台对S.anulatus 89-2-2进行全基因组测序;采用相关软件对菌株89-2-2的基因组序列进行拼接、基因预测、功能注释、进化分析和共线性分析,并预测次级代谢产物合成基因簇,比较不同链霉菌的基因组间差异,研究不同种链霉菌的次级代谢潜力及物种间的进化关系。【结果】菌株89-2-2基因组为一条总长度为8117999 bp的线性染色体,G+C含量为71.52%,序列已提交至NCBI的GenBank数据库,登录号为CP137002。生物信息学分析发现基因组含有7088个编码基因,分别在COG、GO、KEGG、NR数据库提取到5300、4176、2513、7013个基因的注释信息。同时,通过antiSMASH软件预测到34个次级代谢产物合成基因簇,涉及萜烯类、非核糖体肽类、聚酮类、翻译后修饰肽类等多种天然产物的合成,其中11个基因簇与已知化合物编码基因簇同源性较低,说明该菌株具有产生多种新型次级代谢产物的潜力。通过对菌株89-2-2与S.microflavus DSM40593、Streptomyces sp.AM2-1-1基因组比较分析发现,菌株89-2-2与菌株AM2-1-1基因组线性关系较好,具有较近的亲缘关系;而与菌株DSM40593存在较大区域的倒位现象,亲缘关系较远。菌株89-2-2特有的基因家族和基因数目分别为51个和1235个。这些结果表明,链霉菌属具有较强的适应多样生态环境的能力,�[Background]Streptomyces anulatus 89-2-2,a moderately halophilic actinomycete strain,can produce secondary metabolites capable of inhibiting the tyrosinase activity in mushrooms and the melanin synthesis and tyrosinase activity in mouse melanoma B16 cells.Few studies report the genome sequence of S.anulatus 89-2-2,which limits the studies on the biosynthesis and regulation of tyrosinase inhibitors,melanin synthesis inhibitors and other secondary metabolites in the strain.[Objective]This study sequenced the genome of S.anulatus 89-2-2 and mined the genetic resources of secondary metabolites,aiming to lay a foundation for deciphering the mechanisms of tyrosinase inhibitor production and biosynthesis regulation in this strain.[Methods]Nanopore sequencing platform was used to uncover the genome sequence of S.anulatus 89-2-2.Bioinformatics tools were used for sequence assembly,gene prediction,functional annotation,phylogenetic analysis,synteny analysis,and prediction of the gene clusters for biosynthesis of secondary metabolites.The genomes of different Streptomyces spp.were compared,and the secondary metabolic potential and the evolutionary relationship of different Streptomyces spp.were studied.[Results]The genome of strain 89-2-2 was a single linear chromosome with a length of 8117999 bp and the G+C content of 71.52%.The sequence has been submitted to the GenBank of the NCBI,with the accession number CP137002.The genome of the strain contained 7088 coding sequences.The annotation against COG,GO,KEGG,and NR predicted 5300,4176,2513,and 7013 genes,respectively.The antiSMASH predicted 34 gene clusters for the biosynthesis of secondary metabolites in the genome of 89-2-2,and these gene clusters were involved in the biosynthesis of a variety of natural products,such as terpenoids,non-ribosomal peptides,polyketides,and ribosomally synthesized and post-translationally modified peptides.Eleven clusters showed low similarities to the gene clusters for the biosynthesis of known compounds,which suggested that strain 89-2-2 ha

关 键 词：中度嗜盐链霉菌 酪氨酸酶抑制剂 全基因组测序 生物合成基因簇 

分 类 号：Q933[生物学—微生物学]