Identification and functional verification of Y-chromosome-specific gene typo-gyf in Bactrocera dorsalis  

作　　者：Peipei Liu Shuning Yu Wenping Zheng Qiuyuan Zhang Jiao Qiao Ziniu Li Zhurong Deng Hongyu Zhang 

机构地区：[1]National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops,Hubei Hongshan Laboratory,China-Australia Joint Research Centre for Horticultural and Urban Pests,Institute of Urban and Horticultural Entomology,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,China

出　　处：《Insect Science》2024年第4期1270-1284,共15页昆虫科学（英文版）

基　　金：supported by the National Natural Science Foundation of China(no.32220103009);the China Agriculture Research System of MOF and MARA(No.CARS-26)and Hubei Hongshan Laboratory.

摘　　要：Genes on the Y chromosome play important roles in male sex determination and development.The identification of Y-chromosome-specific genes not only provides a theoretical basis for the study of male reproductive development,but also offers genetic control targets for agricultural pests.However,Y-chromosome genes are rarely characterized due to their high repeatability and high heterochromatinization,especially in the oriental fruit fly.In this study,1011 Y-chromosome-specific candidate sequences were screened from 2 to 4 h Bactrocera dorsalis embryo datasets with the chromosome quotient method,6 of which were identified as Y-chromosome-specific sequences by polymerase chain reaction,including typo-gyf,a 19126-bp DNA sequence containing a 575-amino acid open reading frame.Testicular deformation and a significant reduction in sperm number were observed after typo-gyf knockdown with RNA interference in embryos.After typo-gyf knockout with clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9 in the embryonic stage,the sex ratio of the emergent adults was unbalanced,with far more females than males.A genotype analysis of these females with the Y-chromosome gene MoY revealed no sex reversal.Typo-gyf knockout led to the death of XY individuals in the embryonic stage.We conclude that typo-gyf is an essential gene for male survival,and is also involved in testicular development and spermatogenesis.The identification of typo-gyf and its functional verification provide insight into the roles of Y-chromosome genes in male development.

关 键 词：Bactrocera dorsalis SPERMATOGENESIS TESTIS typo-gyf gene Y-specific gene 

分 类 号：Q96[生物学—昆虫学]