三磷酸腺苷生物发光法在提高复杂样品细菌检测能力方面的应用研究进展  

Research progress on the application of adenosine triphosphate bioluminescence method in improving the detection ability of bacteria in complex samples

作　　者：张桐 谢茂梅 刘艺丹 王海霞 ZHANG Tong;XIE Mao-Mei;LIU Yi-Dan;WANG Hai-Xia(College of Pharmaceutical Engineering of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Tianjin Key Laboratory of Chinese Medicine Intelligent Pharmaceutical and Green Pharmaceutical,Tianjin 301617,China;Haihe Laboratory of Modern Chinese Medicine,Tianjin 301617,China)

机构地区：[1]天津中医药大学中药制药工程学院,天津301617 [2]天津市中药智能制药与绿色制药重点实验室,天津301617 [3]现代中医药海河实验室,天津301617

出　　处：《食品安全质量检测学报》2024年第14期1-8,共8页Journal of Food Safety and Quality

基　　金：国家自然科学基金项目(82003944);现代中医药海河实验室科技项目(22HHZYSS00006);2023年天津市高等学校研究生教育改革研究计划项目(TJYG110)。

摘　　要：微生物污染对食品、医药、卫生等领域造成严重危害,因此,开发微生物快速灵敏检测技术至关重要。三磷酸腺苷(adenosine triphosphate,ATP)生物发光法相比于其他微生物检测技术,具有检测速度快、可实时检测等优点。近年来,随着检测技术与设备的不断改进,该技术在复杂样品中的检测应用能力得到了进一步提升。本文系统介绍了ATP生物发光法的原理和局限,从技术和设备层面综述了近年来创新性ATP生物发光技术和新兴检测设备,总结了该技术及设备在复杂样品细菌检测方面的应用现状,以期为提高复杂样品细菌检测能力提供参考。Microbial contamination has caused serious harm to food,medicine,health and other fields,therefore,developing rapid and sensitive microbial detection technologies is crucial.Compared with other microbial detection techniques,adenosine triphosphate(ATP)bioluminescence has the advantages of fast detection speed and real-time detection.In recent years,with the continuous improvement of detection technology and equipment,the detection application ability of this technology in complex samples has been further enhanced.This article systematically introduced the principle and limitations of ATP bioluminescence method,summarized innovative ATP bioluminescence technology and emerging detection equipment in recent years from the technical and equipment levels,and summarized the application status of this technology and equipment in the bacteria detection for complex sample,which hopes to provide reference for improving the detection bacteria ability in complex samples.

关 键 词：三磷酸腺苷生物发光法 微生物检测 检测设备 荧光素酶 

分 类 号：R446.5[医药卫生—诊断学] O657.3[医药卫生—临床医学]