Ⅱ型鼠李半乳糖醛酸聚糖的制备方法和结构表征的研究进展  

Advances in the preparation and structural characterization of rhamnogalacturonan II

作　　者：刘育朋 施松善[1] 步盈萱 龚欢 王辉俊 王顺春[1,2] LIU Yupeng;SHI Songshan;BU Yingxuan;GONG Huan;WANG Huijun;WANG Shunchun(Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203;Key Laboratory of Chinese Medicine Standardization,Ministry of Education,Shanghai 201203,China)

机构地区：[1]上海中医药大学中药研究所,上海201203 [2]中药标准化教育部重点实验室,上海201203

出　　处：《中国药科大学学报》2024年第4期432-442,共11页Journal of China Pharmaceutical University

基　　金：上海市自然科学基金项目(No.21ZR1462900);国家自然科学基金项目(No.82274078,No.U23A20512);上海市东方英才计划青年项目。

摘　　要：Ⅱ型鼠李半乳糖醛酸聚糖(RG-Ⅱ)是果胶结构域中的一种,其结构在不同物种之间具有高度的保守性。RG-Ⅱ的主链由约9个半乳糖醛酸经α-1,4糖苷键连接而成,并被6个(A–F)明确定义的侧链取代。其中二糖侧链C、D和单糖侧链E、F的结构在不同来源的RG-Ⅱ中基本保持相同。寡糖侧链A和B则存在轻微的变异性。通过相对分子质量、单糖组成和质谱分析等手段可实现RG-Ⅱ的结构表征。中药中含有RG-Ⅱ结构域的多糖具有很高的药用价值,使用内切多聚半乳糖醛酸酶(Endo-PG)和草酸青霉可将RG-Ⅱ从中药中分离并对其在多糖中的含量进行测定。从葡萄酒中可快速制备大量RG-Ⅱ标准品用于新定量方法的开发,实现对中药活性多糖的质量控制,推动中药多糖的研究进程。Rhamnogalacturonan II(RG-II)is one of the structural domains of pectin whose structure is highly conserved among species.The main chain of RG-II consists of approximately nine galacturonic acids linked byα-1,4 glycosidic bonds,with six well-defined side chains replacing them(A−F).The structures of the disaccharide side chains C and D and the monosaccharide side chains E and F in RG-II from different sources remain essentially the same.In contrast,the oligosaccharide side chains A and B showed slight variability.Structural characterization of RG-II can be achieved by molecular weight,monosaccharide composition,and mass spectrometry.The polysaccharides containing RG-II structural domains in traditional Chinese medicines(TCMs)have high medicinal value.Isolation of RG-II can be achieved using endo-polygalacturonase(Endo-PG)and Penicillium oxalicum.A substantial number of RG-II standards can be rapidly prepared from red wine for the development of new quantitative methods to realize the quality control of active polysaccharides from traditional Chinese medicines and to promote the research process of polysaccharides from traditional Chinese medicines.

关 键 词：果胶 Ⅱ型鼠李半乳糖醛酸聚糖 RG-Ⅱ 内切多聚半乳糖醛酸酶 草酸青霉 葡萄酒 

分 类 号：R932[医药卫生—生药学]