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作 者:陈佳璇[1] 刘巧谊[1] 张晶[1] 陶霞[1] 陈惠玲[1] 张欣强[1] CHEN Jiaxuan;LIU Qiaoyi;ZHANG Jing;TAO Xia;CHEN Huiling;ZHANG Xinqiang(Guangzhou Center for Disease Control and Prevention,Guangzhou,Guangdong 510440,China)
机构地区:[1]广州市疾病预防控制中心微生物检验部,广东广州510440
出 处:《热带医学杂志》2024年第7期1036-1039,共4页Journal of Tropical Medicine
基 金:广东省医学科学技术研究基金(A2021040);广州市医学重点学科;广州市科技计划项目(2024A03J0420)。
摘 要:目的了解广州地区副溶血性弧菌的血清型、毒力基因和分子特征,为副溶血性弧菌疾病的防控提供科学依据。方法对2020-2022年收集到的副溶血性弧菌菌株进行血清分型、毒力基因检测和脉冲场凝胶电泳(PFGE),并使用国家致病菌识别网的比对功能进行同源性分析。结果收集到132株副溶血性弧菌,其中50.8%(67/132)的菌株血清型为O10:K4,其次为O3:K6(34.8%,46/132);毒力基因结果显示所有的菌株tlh基因阳性(100.0%,132/132),有130株tdh基因阳性(98.5%,130/132),2株trh基因阳性(1.5%,2/132);PFGE聚类分析发现菌株可分为31个PFGE型别,菌株间相似值为23.54%~100.00%,有6个集中聚类。结论广州地区副溶血性弧菌引起的食源性疾病以O10:K4和O3:K6血清型为主,大多数菌株携带tdh基因,PFGE图谱提示基因多样性。Objective To analyze the serotype,virulence genes and gene characteristics of Vibrio parahaemolyticus isolates from Guangzhou,and provide a scientific basis for prevention and control of Vibrio parahaemolyticus.Methods The serotypes,virulence genes and pulse⁃field gel electrophoresis(PFGE)type of all the isolated stains of Vibrio parahaemolyticus were detected during 2020-2022.Homology analysis was performed using the comparison function of the Chinese Pathogen Identification Net.Results O10:K4(50.8%,67/132)and O3:K6(34.8%,46/132)were the predominant serotypes of the 132 Vibrio parahaemolyticus strains isolated.Virulence gene detection indicated all strains were detected positive for tlh genes(100.0%,132/132),130 isolates expressed tdh genes(98.5%,130/132),only 2 isolates expressed trh genes(1.5%,2/132).These strains could be divided into 31 PFGE patterns,and the similarity coefficients were between 23.54%-100.00%.It belonged to 6 clone clusters.Conclusions O10:K4 and O3:K6 were the most dominant serotypes in Vibrio parahaemolyticus strains from foodborne disease in Guangzhou,and tdh was detected in most of the strains.PFGE showed there was great diversity in this gene.
分 类 号:R155.3[医药卫生—营养与食品卫生学]
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