双歧杆菌高密度培养的补料培养基及补料方法  

Supplementary culture medium and supplementationmethod for high-density cultivation of Bifidobacterium

作　　者：季学猛 王硕[1] JI Xuemeng;WANG Shuo(School of Medicine,Nankai University,Tianjin 300071,China)

机构地区：[1]南开大学医学院,天津300071

出　　处：《实验室科学》2024年第4期28-31,共4页Laboratory Science

摘　　要：双歧杆菌在维护宿主健康方面具有重要作用,因此对其高密度培养条件的探索具有重要意义。目前,双歧杆菌的高密度培养主要受到培养基组分和培养条件优化的影响。报道了一种用于双歧杆菌高密度培养的补料培养基及补料方法。该方法使用补料与碱泵耦合的方法进行补料,通过控制发酵培养基的pH值来调节补料培养基的补入量。此外,还进行了补料培养基的优化实验,通过调整氢氧化钠和葡萄糖浓度的比例,比较了不同补料培养基的发酵性能。实验结果表明该补料培养基及补料方法适用于两歧双歧杆菌、青春双歧杆菌、动物双歧杆菌、长双歧杆菌等多种双歧杆菌,而且能够达到较高的活菌密度。提出的补料培养基及补料方法可为双歧杆菌的高密度培养提供有效的解决方案。Bifidobacterium plays a significant role in maintaining host health,making the exploration of high-density cultivation conditions crucial.Currently,the high-density cultivation of Bifidobacterium is mainly influenced by the optimization of culture medium components and cultivation conditions.Here,we report a supplementary culture medium and supplementation method for high-density cultivation of Bifidobacterium.The method utilizes coupling of supplementation with an alkaline pump to control the supplementation rate of the culture medium by adjusting its pH value.Furthermore,optimization experiments of the supplementation culture medium were conducted by varying the ratio of sodium hydroxide to glucose concentrations,comparing the fermentation performance of different supplementation culture media.Experimental results demonstrate that this supplementation culture medium and supplementation method are applicable to various Bifidobacterium strains such as Bifidobacterium bifidum,Bifidobacterium adolescentis,Bifidobacterium animalis,and Bifidobacterium longum,achieving high viable cell densities.The proposed supplementation culture medium and supplementation method in this study offer an effective solution for high-density cultivation of Bifidobacterium.

关 键 词：双歧杆菌 高密度培养 补料培养基 补料方法 碱泵耦合 

分 类 号：G482[文化科学—教育学]