高效液相色谱法分析山楂中的四种黄酮成分  

Analysis of Four Flavonoids in Hawthorn Fruit by HPLC

作　　者：梁国嫔 彭睿妍 曹海燕 阙素云 张清峰[1] LIANG Guopin;PENG Ruiyan;CAO Haiyan;QUE Suyun;ZHANG Qingfeng(Jiangxi Key Laboratory of Natural Product and Functional Food,College of Food Science and Engineering,Jiangxi Agriculture University,Nanchang 330045,China;Jiangxi Drug Inspection Center,Ji'an 331500,China;Jiangxi Weilaiyingjian High-tech Co.,Ltd.,Ji'an 331500,China)

机构地区：[1]江西农业大学食品科学与工程学院,江西省天然产物与功能食品重点实验室,南昌330045 [2]江西省药品检查员中心,吉安331500 [3]江西维莱营健高科有限公司,吉安331500

出　　处：《食品与发酵科技》2024年第4期104-108,共5页Food and Fermentation Science & Technology

基　　金：国家自然科学基金项目(32060541)。

摘　　要：建立了山楂中四种黄酮成分(芦丁、牡荆素、金丝桃苷和槲皮素)的高效液相色谱分析方法。采用Agilent 5 TC-C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈(A)和0.2%醋酸水溶液(B),流速为1 mL/min,进样量为10μL,检测波长为355nm,柱温为40℃。线性梯度洗脱程序:0~15min,20%~30%A;15~30min,30%~60%A。四种成分线性关系良好;日内精密度保留时间RSD均<0.5%,峰面积RSD均<2.5%;日间精密度保留时间RSD均<0.5%,峰面积RSD均<5.0%;平均加样回收率为103.4%~106.7%,RSD均<2.5%。60%乙醇为最佳提取溶剂。6批山楂样品中芦丁和金丝桃苷的含量范围分别为51.8~264.5μg/g和148.0~441.6μg/g,槲皮素只在两批样品中检出,分别为11.0μg/g和12.7μg/g,牡荆素在所有山楂样品中均未检出。A HPLC method was developed to determine four flavonoids in hawthorn fruit,including rutin,vitexin,hypericin and quercetin.An Agilent 5 TC-C18 column(250 mm×4.6 mm,5μm)was used with the mobile phases consisting of acetonitrile(A)and 0.2%acetic acid(B)at the flow rate of 1 mL/min.The injection volume was 10μL,while the detection wavelength was set at 355 nm and the column temperature was 40℃.The linear gradient elution procedure was 20%~30%A in 0~15 min,and 30%~60%A in 15~30 min.Methodological results showed that the four components have a good linear relationship.The intraday precision retention time RSD was<0.5%,and the peak area RSD was<2.5%.The RSD of interday precision retention time was<0.5%and the RSD of peak area was<5.0%.The average recoveries ranged from 103.4%to 106.7%,and the RSD was<2.5%.The optimal extraction solvent was 60%ethanol.The contents of rutin and hypericin in 6 batches of hawthorn samples were in the range of 51.8~264.5μg/g and 148.0~441.6μg/g,respectively.Quercetin was detected in 2 batches of hawthorn samples,with contents of 11.0 and 12.7μg/g,and vitexin was undetectable in all samples.

关 键 词：山楂 高效液相色谱法 黄酮 

分 类 号：TS207.3[轻工技术与工程—食品科学]