塑料包装材料表面大肠杆菌污染的荧光高光谱检测  

Fluorescence Hyperspectral Detection of Escherichia coli Contamination on the Surface of Plastic Packaging Materials

作　　者：熊子怡 潘嘹 卢立新 唐亚丽 王军[1,2] XIONG Ziyi;PAN Liao;LU Lixin;TANG Yali;WANG Jun(School of Mechanical Engineering,Jiangnan University,Wuxi 214122,China;Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment and Technology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学机械工程学院,江苏无锡214122 [2]江苏省食品先进制造装备技术重点实验室,江苏无锡214122

出　　处：《食品科学》2024年第18期225-231,共7页Food Science

基　　金：江苏省食品先进制造装备技术重点实验室自主研究课题项目(FMZ202202)。

摘　　要：本研究基于荧光高光谱技术,采用365 nm波段紫外光作为激发光源,获得塑料包装表面421~700 nm波段范围内的荧光高光谱图像,确定多种塑料包装材料(聚乙烯、聚丙烯、聚酰胺)表面大肠杆菌污染的荧光特征光谱,并确定生物膜检测限。结果表明,聚乙烯、聚丙烯和聚酰胺表面大肠杆菌荧光光谱特征波长分别位于550、543、540 nm;基于上述特征波长处的单波段图像经过处理能够准确检出3种塑料包装材料表面的大肠杆菌接种点,检出率均大于98%;对聚乙烯、聚丙烯和聚酰胺表面大肠杆菌生物膜的检测限分别为1.39、1.39(lg(CFU/cm^(2)))和1.59(lg(CFU/cm^(2)))。本研究可为包装食品微生物检测及食品安全监控提供有力的技术手段和理论支持。In this study,the fluorescence hyperspectral images of plastic packaging surfaces in the wavelength range of 421–700 nm were collected using fluorescence hyperspectral imaging(F-HSI)technique with ultraviolet(UV)light at 365 nm as the excitation light source.The characteristic fluorescence spectra of Escherichia coli contamination on the surface of various plastic packaging materials(polyethylene(PE),polypropylene(PP),and polyamide(PA))were determined and the detection limit for the biofilm of E.coli was established.The results showed that the characteristic fluorescence wavelengths of E.coli on PE,PP and PA surfaces were 550,543,and 540 nm,respectively.Based on the processed single-band images at these characteristic wavelengths,E.coli inoculation points on the surface of the three plastic packaging materials could be accurately detected,and the detection rates were all greater than 98%.The detection limits of E.coli biofilm on PE,PP and PA surfaces were 1.39,1.39 and 1.59(lg(CFU/cm^(2))),respectively.The research results provide a powerful technical means and theoretical support for microbiological detection of packaged foods and food safety monitoring.

关 键 词：荧光高光谱 塑料包装材料 大肠杆菌 图像处理 生物膜 

分 类 号：TS206.6[轻工技术与工程—食品科学]