同位素内标-超高效液相色谱-串联质谱法快速测定食品中米酵菌酸及异米酵菌酸  

Rapid determination of bongkrekic acid and iso-bongkrekic acid in food by UPLC-MS/MS with isotope internal standards

作　　者：李昌安[1] 章剑[1] 张维 张晨惠 朱国勋 LI Chang'an;ZHANG Jian;ZHANG Wei;ZHANG Chenhui;Zhu Guoxun(Hefei Centers for Disease Control and Prevention,Hefei,230061,China)

机构地区：[1]合肥市疾病预防控制中心,合肥230061

出　　处：《化学分析计量》2024年第8期24-31,共8页Chemical Analysis And Meterage

基　　金：合肥市卫生健康委应用医学研究重点项目(Hwk2022zc047)。

摘　　要：建立一种快速测定不同食品中米酵菌酸和异米酵菌酸的超高效液相色谱-串联质谱联用方法。样品中待测物用含0.1%氨水的甲醇-水溶液(体积比为4∶1)提取后,经过MFC335柱净化,BEH C_(18)色谱柱分离,负离子扫描,多反应监测模式检测,同位素内标法定量。两种待测物在0.5~200μg/L质量浓度范围内线性良好,相关系数均大于0.995,检出限和定量限分别为0.2、0.6μg/kg,平均回收率范围为90.6%~109.2%,测定结果的相对标准偏差为2.0%~11.2%(n=6)。该方法适用于各类食品基质中米酵菌酸和异米酵菌酸的快速筛查与公共卫生应急检测。A method for rapid determination of bongkrekic acid(BA)and iso-bongkrekic acid(IBA)in different food by ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)was established.The BA and IBA in food were extracted with methanol water solution(the volume ratio was 4∶1)containing 0.1%ammonia solution and cleaned up with MFC335 column and then separated on BEH C_(18) chromatographic column,monitored with multiple reactions monitoring(MRM)mode under negative scanning(ESI-),and finally quantified by isotope internal standard method.The BA and IBA showed good linearity in the range of 0.5-200μg/L with the correlation coefficients all exceeding 0.995.The limits of detection and limits of quantification were 0.2μg/kg and 0.6μg/kg,respectively.The average recoveries ranged from 90.6%to 109.2%,and the relative standard deviations were 2.0%-11.2%(n=6).The method is suitable for rapid screening and public health emergency testing of BA and IBA in various food matrices.

关 键 词：超高效液相色谱-串联质谱法 米酵菌酸 异米酵菌酸 食品 同位素内标 

分 类 号：O657[理学—分析化学]