茶树海藻糖-6-磷酸合成酶(TPS)基因家族鉴定与表达分析  

Genome-wide Identification and Expression Pattern Profiling of the Trehalose-6-phosphate Synthase(TPS)Gene Family in Tea Plant(Camellia sinensis)

作　　者：刘丹丹 王雷刚 孙明慧 焦小雨 吴琼 王文杰 LIU Dan-dan;WANG Lei-gang;SUN Ming-hui;JIAO Xiao-yu;WU Qiong;WANG Wen-jie(Tea Research Institute,Anhui Academy of Agricultural Sciences,Hefei 230001)

机构地区：[1]安徽省农业科学院茶叶研究所,合肥230001

出　　处：《生物技术通报》2024年第8期152-163,共12页Biotechnology Bulletin

基　　金：国家自然科学基金联合基金项目(U23A20213);安徽省茶叶产业技术体系(AHCYJSTX-11);安徽省农业科学院成果转化项目(2024YL041)。

摘　　要：【目的】海藻糖-6-磷酸合成酶(trehalose-6-phosphate synthase,TPS)是植物体内海藻糖生物合成通路中的关键酶。对茶树[Camellia sinensis(L.)O.Kuntze]TPS基因家族进行鉴定和分析,并研究其在逆境胁迫下的表达模式,为开展TPS基因功能验证提供基础。【方法】利用生物信息学方法鉴定茶树CsTPS基因家族成员,分析其编码的蛋白理化特性、保守基序与结构域、染色体定位、系统进化关系和启动子顺式作用元件等生物学信息,利用转录组数据结合RT-qPCR技术分析CsTPS家族成员在干旱、盐和低温胁迫下的表达模式。【结果】在茶树基因组中鉴定到16个TPS基因家族成员,系统发育分析将它们划分为Class I和Class II两个亚族。同一亚族成员具有相似的motif组成和基因结构。共线性与进化分析表明,CsTPS基因在茶树基因组内发生了基因复制事件,且它们在进化过程中经历了纯化选择作用。RT-qPCR结果显示,6个候选基因CsTPS3、CsTPS5、CsTPS6、CsTPS9、CsTPS11和CsTPS14均被PEG、NaCl和低温胁迫诱导,且表达模式与转录组结果一致。【结论】从茶树全基因组中系统鉴定出16个TPS家族成员。茶树CsTPS3、CsTPS5、CsTPS6、CsTPS9、CsTPS11和CsTPS14基因对干旱、盐和低温胁迫均有响应,但其敏感性水平各不相同。【Objective】Trehalose-6-phosphate synthase(TPS)is a key enzyme in the trehalose biosynthetic pathway in plants.The TPS gene family of the tea plant[Camellia sinensis(L.)O.Kuntze]was identified,analyzed,and its expression pattern under adverse stress was studied.This study aims to provide a foundation for the functional validation of TPS genes.【Method】Utilizing biological information methods,we identified members of CsTPS gene family and analyzed the physical and chemical properties of their encoded protein,conserved motifs and domains,chromosomal location,phylogenetic relationships,promoter cis-acting elements,and other biological information.Subsequently,we employed transcriptome data in conjunction with RT-qPCR technology to investigate the expression patterns of CsTPS family members under drought,salt,and cold stress.【Result】The 16 TPS gene family members were identified in C.sinensis genome,which were classified into two subfamilies:Class I and Class II based on phylogenetic study.Members of the same subfamily presented similar motif composition and gene structure.The covariance and evolutionary studies revealed that CsTPS genes were duplicated within the tea plant genome and experienced purifying selection during the evolutionary process.RT-qPCR results revealed that the six candidate genes,CsTPS3,CsTPS5,CsTPS6,CsTPS9,CsTPS11,and CsTPS14,were all induced by PEG,NaCl,and low-temperature stress,and their expression patterns were similar with transcriptome information.【Conclusion】Sixteen TPS family members were identified systematically across the tea plant's genome.The CsTPS3,CsTPS5,CsTPS6,CsTPS9,CsTPS11,and CsTPS14 genes all are sensitive to drought,salt,and low-temperature stresses,though their sensitivity levels differed.

关 键 词：茶树 海藻糖-6-磷酸合成酶基因 系统进化 表达模式 非生物胁迫 

分 类 号：Q943.2[生物学—植物学] S571.1[农业科学—茶叶生产加工]