Single-nucleus transcriptomics uncovers a geroprotective role of YAP in primate gingival aging  

作　　者：Qinchao Hu Bin Zhang Yaobin Jing Shuai Ma Lei Hu jingyi Li Yandong Zheng Zijuan Xin Jianmin Peng Si Wang Bin Cheng Jing Qu Weiqi zhang Guang-Hui Liu Songlin Wang 

机构地区：[1]State Key Laboratory of Membrane Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China [2]Hospital of Stomatology,Guanghua School of Stomatology,Guangdong Provincial Key Laboratory of Stomatology,Sun Yat-sen University,Guangzhou 510060,China [3]Salivary Gland Disease Center and Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction,Beijing Laboratory of Oral Health and Beijing Stomatological Hospital,Capital Medical University,Beijing 100050,China [4]Department of Biochemistry and Molecular Biology,School of Basic Medical Sciences,Capital Medical University,Beijing 100069,China [5]CAS Key Laboratory of Genomic and Precision Medicine,Beijing Institute of Genomics,Chinese Academy of Sciences and China National Center for Bioinformation,Beijing 100101,China [6]State Key Laboratory of Stem Cell and Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China [7]Key Laboratory of Organ Regeneration and Reconstruction,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China [8]University of Chinese Academy of Sciences,Beijing 100049,China [9]Institute for Stem Cell and Regeneration,Chinese Academy of Sciences,Beijing 100101,China [10]Beijing Institute for Stem Cell and Regenerative Medicine,Beijing 100101,China [11]Advanced Innovation Center for Human Brain Protection and National Clinical Research Center for Geriatric Disorders,Xuanwu Hospital Capital Medical University,Beijing 100053,China [12]Aging Translational Medicine Center,Intermational Center for Aging and Cancer,Beijing Municipal Geriatric Medical Research Center,Xuan Wu Hospital,Capital Medical University,Beijing 100053,China [13]Intemational Center for Aging and Cancer,Hainan Medical University,Haikou 571199,China [14]Aging Biomarker Consortium,Beijing 100101,China

出　　处：《Protein & Cell》2024年第8期612-632,共21页蛋白质与细胞（英文版）

摘　　要：Aging has a profound impact on the gingiva and significantly increases its susceptibility to periodontitis,a worldwide prevalent inflammatory disease.However,a systematic characterization and comprehensive understanding of the regulatory mechanism underlying gingival aging is still lacking.Here,we systematically dissected the phenotypic characteristics of gingiva during aging in primates and constructed the first single-nucleus transcriptomic landscape of gingival aging,by which a panel of cell type-specific signatures were elucidated.Epithelial cells were identified as the most affected cell types by aging in the gingiva.Further analyses pinpointed the crucial role of YAP in epithelial self-renew and homeostasis,which declined during aging in epithelial cells,especially in basal cells.The decline of YAP activity during aging was confrmed in the human gingival tissues,and downregulation of YAP in human primary gingival keratinocytes recapitulated the major phenotypic defects observed in the aged primate gingiva while overexpression of YAP showed rejuvenation effects.Our work provides an in-depth understanding of gingival aging and serves as a rich resource for developing novel strategies to combat aging-associated gingival diseases,with the ultimate goal of advancing periodontal health and promoting healthy aging.

关 键 词：single-nucleus RNA-sequencing PRIMATE GINGIVA AGING YAP 

分 类 号：Q75[生物学—分子生物学]