缺氧微环境对转移性皮肤黑色素瘤预后及免疫治疗的影响  

Effect of hypoxic microenvironment on prognosis and immunotherapy of metastatic cutaneous melanoma

作　　者：杨文超 秦瑞宁 张俊瑕 YANG Wenchao;QIN Ruining;ZHANG Junxia(Department of Public Health,Zhangye People's Hospital Affliated to Hexi University,Zhangye 734000,China)

机构地区：[1]河西学院附属张掖人民医院公卫科,甘肃张掖734000

出　　处：《中国美容整形外科杂志》2024年第8期471-476,共6页Chinese Journal of Aesthetic and Plastic Surgery

摘　　要：目的建立一种基于缺氧基因模型来评估皮肤黑色素瘤(skin cutaneous melanoma,SKCM)预后和免疫微环境.方法自2023年3-7月,河西学院附属张掖人民医院公卫科通过检索公共数据库(TCGA、GEO、GTEx)查找SKCM病例,Cox回归分析筛选缺氧相关性状的基因.构建了一个包含7个缺氧基因的风险模型.GSEA用于比较两组的富集差异,使用CIBERSORT工具研究免疫细胞和基因特征之间潜在的相关性.结果发现SKCM缺氧模型含有的7个基因(FBP1、GPI、BGN、SERPINE1、SDC4、PGM1、EGFR)高风险组患者的存活率低于低风险组.此外,9种免疫细胞在高、低风险组患者的组织中有明显的浸润差异.结论缺氧与SKCM患者的预后和免疫细胞浸润的发生率相关,这为个性化治疗的发展提供了免疫学视角.Objective To develop a hypoxia-based gene model to assess prognosis and immune microenvironment of skin cuta-neous melanoma(SKCM).Methods Public database(TCGA,GEO,GTEx)was used to search SKCM cases.Cox regression analysis was used to screen genes for hypoxia-related traits.A risk model containing seven hypoxia genes was constructed.GSEA was used to com-pare enrichment differences between the two groups,using the CIBERSORT tool to investigate potential correlations between immune cells and genetic signatures.Results Seven genes(FBP1,GPI,BGN,SERPINE1,SDC4,PGM1,EGFR)contained in the SKCM hypoxia model were found to have a lower survival rate in the high-risk group than in the low-risk group.In addition,we found significant differ-ences in the infiltration of nine immune cells in the tissues of patients in the high-and low-risk groups.Conclusion Hypoxia is associated with the prognosis and the incidence of immune cell infiltration in SKCM patients,which provides an immunological perspective for the de-velopment of personalized therapy.

关 键 词：皮肤黑色素瘤 缺氧 免疫治疗 公共数据库 生物信息学 

分 类 号：R739.5[医药卫生—肿瘤]