基于HS-SPME-GC/MS对湖景蜜露和白丽桃香气成分分析  

HS-SPME-GC/MS analysis of aroma components in‘Hujingmilu'and‘Baili'peach

作　　者：吕靖芳 黄雅丽 戴芬[2] 宋欣悦 朱作艺[2] LU Jingfang;HUANG Yali;DAI Fen;SONG Xinyue;ZHU Zuoyi(Agricultural Product Quality and Safety Testing Center of Shaoxing Shangyu District,Zhejiang 312300;Agriculture Product Quality Safety and Nutrition Institute,Zhejiang Academy of Agricultural Sciences;College of Chemical Engineering,Zhejiang University of Technology)

机构地区：[1]绍兴市上虞区农产品质量安全检测中心,浙江312300 [2]浙江省农业科学院农产品质量安全与营养研究所 [3]浙江工业大学化学工程学院

出　　处：《中国果树》2024年第8期24-31,38,共9页China Fruits

基　　金：浙江省农业科学院地方科技合作项目(SY202309)。

摘　　要：香气是评价果实品质的重要指标之一,果实中不同香气物质的构成使果实呈现独特的风味。以中晚熟水蜜桃品种湖景蜜露和白丽作为试验对象,采用顶空固相微萃取技术结合气相色谱-质谱联用(HS-SPME-GC/MS)对两个水蜜桃品种的挥发性香气成分进行提取和分析鉴定。结果表明,白丽桃果实中的挥发性物质相对含量显著高于湖景蜜露桃果实,在两个水蜜桃品种中均检测到己醛、己烯醛、己醇和己烯醇等具有特征青香成分的C_(6)醛类和C_(6)醇类物质,以及乙酸己酯、γ-己内酯、R-γ-癸内酯、δ-癸内酯、γ-十二碳内酯等具有特征果香成分的酯类物质。此外,结合香气阈值计算各香气物质的香气活性值(OAV),湖景蜜露桃和白丽桃的香气成分中,分别鉴定出10种和15种平均OAV>1的香气活性成分,其中正己醛具有较高的OAV,且在白丽桃中显著高于湖景蜜露桃;而湖景蜜露桃中的芳樟醇OAV高于大部分白丽桃。Aroma is one of the important indexes to evaluate fruit quality,the composition of different aroma substances in the fruit makes the fruit show a unique flavor.In this study,the middle and late mature peach varieties‘Hujingmilu'and‘Baili'were used as experimental objects,and the volatile aroma components of the two peach varieties were extracted,analyzed and identified by head space solid phase microextraction and gas chromatography coupled to mass spectrometry(HS--SPME-GC/MS).The results showed that the relative content of volatile compounds in‘Baili'peach fruits was significantly higher than that in‘Hujingmilu'peach fruits.However,C_(6) aldehydes and C_(6) alcohols with characteristic green incense components such as hexanal,hexenal,hexanol and hexenol were detected in the two peach varieties,and esters with characteristie fruit flavor components,such as hexyl acetate,Y-caprolactone,R-y-decanolactone,8-decalactone and r-dodecanolide.In addition,the aroma activity value(0AV)of each aroma substance was calculated according to the aroma threshold.Among the aroma components of‘Hujingmilu'fruits and‘Baili'fruits,10 and 13 kinds of aroma active components with 0AV>1 were identified,respectively.The OAV of hexanal were relatively high,and it was significantly higher in the‘Baili'peach fruits than in the‘Hujingmilu'peach fruits;the OAV of linalool in‘Hujingmilu’peach fruits was higher than that in most‘Baili'peach fruits.

关 键 词：桃 湖景蜜露 白丽 香气成分 顶空固相微萃取-气质联用 

分 类 号：S662.1[农业科学—果树学]