海南草豆蔻挥发油化学成分分析及对单增李斯特菌抑菌性的研究  

Analysis of Chemical Components of Volatile Oil from Hainan Alpinia katsumadai Hayata and Study on Its Antimicrobial Activity Against Listeria monocytogenes

作　　者：王丹[1] 丁书仙[1] 江芊[1] 汪雪凤 王清隆[1] 王茂媛[1] 陈晓鹭 赵建平 于福来[1] WANG Dan;DING Shu-xian;JIANG Qian;WANG Xue-feng;WANG Qing-long;WANG Mao-yuan;CHEN Xiao-lu;ZHAO Jian-ping;YU Fu-lai(Tropical Crops Germplasm Resources Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Cultivation of Chinese Herbal Medicines,Ministry of Agriculture and Rural Affairs/Hainan Provincial Engineering Research Center for Tropical Medicinal Plants,Haikou 571101,China)

机构地区：[1]中国热带农业科学院热带作物品种资源研究所/农业农村部中药材生物学与栽培重点实验室/海南省热带药用植物工程研究中心,海口571101

出　　处：《中国调味品》2024年第9期89-95,共7页China Condiment

基　　金：海南省重点研发计划项目(ZDYF2024SHFZ132);中国热带农业科学院热带作物品种资源研究所非营利项目(PZS2023013);中国热带农业科学院基本科研业务费专项资金(1630032022008)。

摘　　要：分析海南草豆蔻挥发油的化学成分种类和含量,明确挥发油对单增李斯特菌的抑菌活性。采用水蒸气蒸馏法提取草豆蔻挥发油,利用气相色谱-质谱联用技术(GC-MS)检测和分析其化学成分。采用滤纸片扩散法测定单增李斯特菌对挥发油的药物敏感性,采用微量二倍稀释法测定最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。结果表明,草豆蔻挥发油得率为1.26%,分离鉴定出107种化合物,草豆蔻挥发油对单增李斯特菌的抑菌圈直径为15.44 mm,为高敏感性,MIC和MBC分别为0.078,0.3125 mg/mL。海南草豆蔻挥发油得率较高,挥发性化学成分种类丰富,并且对单增李斯特菌具有较好的抑菌效果。该研究为优异草豆蔻品种的选育提供了数据支持,同时也为草豆蔻挥发油在天然食品抗菌剂开发中的应用提供了理论基础和参考。The types and content of chemical components of volatile oil from Hainan Alpinia katsumadai Hayata are analyzed,and the antimicrobial activity of volatile oil against Listeria monocytogenes is determined.The volatile oil is extracted from Alpinia katsumadai Hayata by steam distillation method and its chemical components are detected and analyzed by gas chromatography-mass spectrometry(GC-MS).The drug sensitivity of Listeria monocytogenes to volatile oil is determined by filter paper diffusion method,and the minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)are determined by trace double dilution method.The results show that the yield of volatile oil from Alpinia katsumadai Hayata is 1.26%,and 107 compounds are isolated and identified.The inhibitory zone diameter of volatile oil against Listeria monocytogenes is 15.44 mm,indicating that it has high sensitivity.MIC and MBC are 0.078,0.3125 mg/mL respectively.The yield of volatile oil from Hainan Alpinia katsumadai Hayata is higher,the types of volatile chemical components are rich,and it has a good antimicrobial effect on Listeria monocytogenes.This study has provided data support for the breeding of excellent Alpinia katsumadai Hayata varieties,and also provided theoretical basis and references for the application of volatile oil from Alpinia katsumadai Hayata in the development of natural food antimicrobial agents.

关 键 词：草豆蔻 海南 挥发油 化学成分 GC-MS 单增李斯特菌 抑菌活性 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]