石莼类菌孢素氨基酸的提取工艺及对副溶血弧菌的抑菌机理  

Research of Extraction Process of Mycosporine-like Amino Acids of Ulva lactuca and Its Mechanism of Anti-bacterial Properties Against Vibrio parahaemolyticus

作　　者：胡晓琪 徐沈晨 王思宇 浦孟宣 胥珍红 马骁 孙颖颖 HU Xiaoqi;XU Shenchen;WANG Siyu;PU Mengxuan;XU Zhenhong;MA Xiao;SUN Yingying(Jiangsu Key Laboratory of Marine Bioresources and Eco-environment,Jiangsu Ocean University,Lianyungang 222005,China;A Co-Innovation Center of Jiangsu Marine Bio-industry Technology,Jiangsu Ocean University,Lianyungang 222005,China)

机构地区：[1]江苏海洋大学,江苏省海洋生物资源与环境重点实验室,江苏连云港222005 [2]江苏海洋大学,江苏省海洋生物产业技术协同创新中心,江苏连云港222005

出　　处：《食品工业科技》2024年第18期175-183,共9页Science and Technology of Food Industry

基　　金：江苏省自然科学研究面上项目(BK20211353);连云港市科技项目重点研发计划(CG2226);江苏海洋大学大学生创新训练计划项目。

摘　　要：为探明石莼类菌孢素氨基酸(MAAs)的提取工艺及对副溶血弧菌的抑菌机理,本研究采用单因素实验和响应面优化试验,分析了液固比、提取温度和提取时间对石莼MAAs得率的影响,并对MAAs进行了硅胶柱层析分离以及HPLC和ESI-MS鉴定。通过扫描、荧光显微镜观察胞内核酸、蛋白质的泄露及DNA分解等分析MAAs对副溶血弧菌的抑菌机理。结果表明,石莼MAAs的最佳提取参数为液固比25:1 mL/g、提取温度40℃和提取时间118 min,制备到的MAAs得率为179.34±3.91 mg/g。石莼MAAs明显破坏了副溶血弧菌的细胞膜,导致了胞内核酸和蛋白质泄露,并促使菌体内DNA发生分解。MAAs经硅胶柱层析分离获得2个组分,经鉴定组分H2为shinorine,其在石莼干粉末中含量可达10.32 mg/g。本研究为石莼MAAs的制备和副溶血弧菌的生物防治提供了技术参考。To explore the extraction process of mycosporine amino acids(MAAs)and the antibacterial mechanism of Vibrio parahaemolyticus,the effects of liquid-solid ratio,extraction temperature and extraction time on the yield of MAAs were analyzed by single factor and response surface optimization tests,and MAAs were isolated and identified by silica gel column chromatography,HPLC and ESI-MS.The antibacterial mechanism of MAAs against Vibrio parahaemolyticus was analyzed by scanning and fluorescence microscopy to observe the leakage of intracellular nucleic acids and proteins,and DNA decomposition.The results showed that the optimal extraction parameters of MAAs of Ulva lactuca was liquid-solid ratio of 25 mL/g,extraction temperature of 40℃and extraction time of 118 min,and the yield of MAAs was 179.34±3.91 mg/g.MAAs significantly destroyed the cell membrane of Vibrio parahaemolyticus,causing the leakage of nucleic acid and proteins,and promoting the breakdown of DNA in the bacteria.MAAs were isolated by silica gel column chromatography to obtain two fractions,and the fraction H2 was identified as shinorine,and the content in Ulva lactuca dry powder could reach 10.32 mg/g.This study provides a technical reference for the preparation of MAAs of Ulva lactuca and the biological control of Vibrio parahaemolyticus.

关 键 词：石莼 类菌孢素氨基酸 提取工艺 副溶血弧菌 抑菌机制 

分 类 号：TS201.2[轻工技术与工程—食品科学]